ArticleMolecular systems biology2009
Analysis of the mitotic exit control system using locked levels of stable mitotic cyclin.
Article in Molecular systems biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 33 citations in OpenAlex.
- Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor.Nature communications · 2023Article
- Quantitative model of eukaryotic Cdk control through the Forkhead CONTROLLER.NPJ systems biology and applications · 2021Review
- Hog1 activation delays mitotic exit via phosphorylation of Net1.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cellular response upon proliferation in the presence of an active mitotic checkpoint.Life science alliance · 2019Article
- Interregulation of CDKA/CDK1 and the Plant-Specific Cyclin-Dependent Kinase CDKB in Control of the Chlamydomonas Cell Cycle.The Plant cell · 2018Article
- Oscillatory stimuli differentiate adapting circuit topologies.Nature methods · 2017Article
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- Article
- Model-Based Analysis of Cell Cycle Responses to Dynamically Changing Environments.PLoS computational biology · 2016Article
- The Chlamydomonas cell cycle.The Plant journal : for cell and molecular biology · 2015Review
- Adaptation to the spindle checkpoint is regulated by the interplay between Cdc28/Clbs and PP2ACdc55.The Journal of cell biology · 2013Article
- Punctuated cyclin synthesis drives early embryonic cell cycle oscillations.Molecular biology of the cell · 2012Article
- Article
- Cdc5-dependent asymmetric localization of bfa1 fine-tunes timely mitotic exit.PLoS genetics · 2012Article
- A quantitative model for cyclin-dependent kinase control of the cell cycle: revisited.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Computational modelling of mitotic exit in budding yeast: the role of separase and Cdc14 endocycles.Journal of the Royal Society, Interface · 2011Article
- Oscillatory dynamics of cell cycle proteins in single yeast cells analyzed by imaging cytometry.PloS one · 2011Article
- Rising cyclin-CDK levels order cell cycle events.PloS one · 2011Article
- Frequency control of cell cycle oscillators.Current opinion in genetics & development · 2010Review
- A virtual cycle: theory and experiment converge on the exit from mitosis.F1000 biology reports · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Cyclin-dependent kinase (Cdk) both promotes mitotic entry (spindle assembly and anaphase) and inhibits mitotic exit (spindle disassembly and cytokinesis), leading to an elegant quantitative hypothesis that a single cyclin oscillation can function as a ratchet to order these events. This ratchet is at the core of a published ODE model for the yeast cell cycle. However, the ratchet model requires appropriate cyclin dose-response thresholds. Here, we test the inhibition of mitotic exit in budding yeast using graded levels of stable mitotic cyclin (Clb2). In opposition to the ratchet model, stable levels of Clb2 introduced dose-dependent delays, rather than hard thresholds, that varied by mitotic exit event. The ensuing cell cycle was highly abnormal, suggesting a novel reason for cyclin degradation. Cdc14 phosphatase antagonizes Clb2-Cdk, and Cdc14 is released from inhibitory nucleolar sequestration independently of stable Clb2. Thus, Cdc14/Clb2 balance may be the appropriate variable for mitotic regulation. Although our results are inconsistent with the aforementioned ODE model, revision of the model to allow Cdc14/Clb2 balance to control mitotic exit corrects these discrepancies, providing theoretical support for our conclusions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.