ArticleNucleic acids research2010
A single-molecule assay for telomerase structure-function analysis.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 36 citations in OpenAlex.
- Strategies for Overcoming the Single-Molecule Concentration Barrier.ACS measurement science au · 2023Review
- Single-Run Catalysis and Kinetic Control of Human Telomerase Holoenzyme.Advances in experimental medicine and biology · 2022Review
- Article
- Single-Molecule Analysis of Reverse Transcriptase Enzymes.Cold Spring Harbor perspectives in biology · 2019Review
- Telomere DNA G-quadruplex folding within actively extending human telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single-Molecule Studies of Telomeres and Telomerase.Annual review of biophysics · 2017Review
- Single-molecule FRET-Rosetta reveals RNA structural rearrangements during human telomerase catalysis.RNA (New York, N.Y.) · 2017Article
- The telomerase essential N-terminal domain promotes DNA synthesis by stabilizing short RNA-DNA hybrids.Nucleic acids research · 2015Article
- The lighthouse at the end of the chromosome.F1000Research · 2015Review
- Unraveling secrets of telomeres: one molecule at a time.DNA repair · 2014Review
- Coordinated DNA dynamics during the human telomerase catalytic cycle.Nature communications · 2014Article
- Single-molecule fluorescence resonance energy transfer studies of the human telomerase RNA pseudoknot: temperature-/urea-dependent folding kinetics and thermodynamics.The journal of physical chemistry. B · 2014Article
- Visualizing cellular machines with colocalization single molecule microscopy.Chemical Society reviews · 2014Review
- Progress in structural studies of telomerase.Current opinion in structural biology · 2014Review
- Single-molecule kinetics reveal cation-promoted DNA duplex formation through ordering of single-stranded helices.Biophysical journal · 2013Article
- A conserved motif in Tetrahymena thermophila telomerase reverse transcriptase is proximal to the RNA template and is essential for boundary definition.The Journal of biological chemistry · 2013Article
- Article
- Roles of telomerase reverse transcriptase N-terminal domain in assembly and activity of Tetrahymena telomerase holoenzyme.The Journal of biological chemistry · 2012Article
- Parallel multispot smFRET analysis using an 8-pixel SPAD array.Proceedings of SPIE--the International Society for Optical Engineering · 2012Article
- Functional importance of telomerase pseudoknot revealed by single-molecule analysis.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The activity of the telomerase ribonucleoprotein enzyme is essential for the maintenance of genome stability and normal cell development. Despite the biomedical importance of telomerase activity, detailed structural models for the enzyme remain to be established. Here we report a single-molecule assay for direct structural analysis of catalytically active telomerase enzymes. In this assay, oligonucleotide hybridization was used to probe the primer-extension activity of individual telomerase enzymes with single nucleotide sensitivity, allowing precise discrimination between inactive, active and processive enzyme binding events. FRET signals from enzyme molecules during the active and processive binding events were then used to determine the global organization of telomerase RNA within catalytically active holoenzymes. Using this assay, we have identified an active conformation of telomerase among a heterogeneous population of enzymes with distinct structures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.