ArticleCirculation. Cardiovascular imaging2009
Molecular MRI of cardiomyocyte apoptosis with simultaneous delayed-enhancement MRI distinguishes apoptotic and necrotic myocytes in vivo: potential for midmyocardial salvage in acute ischemia.
Article in Circulation. Cardiovascular imaging, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Guidelines for measuring cardiac physiology in mice.American journal of physiology. Heart and circulatory physiology · 2018Guideline
- Resistin-like molecule γ attacks cardiomyocyte membranes and promotes ventricular tachycardia.Science (New York, N.Y.) · 2025Article
- Article
- Biomedical Imaging in Experimental Models of Cardiovascular Disease.Circulation research · 2022Review
- CMR for myocardial characterization in ischemic heart disease: state-of-the-art and future developments.European radiology experimental · 2021Review
- Nanoparticle delivery of cardioprotective therapies.Conditioning medicine · 2020Article
- Molecular Imaging of the Heart.Comprehensive Physiology · 2019Review
- Targeting Oxidative Stress Using Nanoparticles as a Theranostic Strategy for Cardiovascular Diseases.Antioxidants & redox signaling · 2019Review
- Molecular Imaging in Ischemic Heart Disease.Current cardiovascular imaging reports · 2019Review
- Annexin A5 reduces infarct size and improves cardiac function after myocardial ischemia-reperfusion injury by suppression of the cardiac inflammatory response.Scientific reports · 2018Article
- Multiplexed Optical Imaging of Energy Substrates Reveals That Left Ventricular Hypertrophy Is Associated With Brown Adipose Tissue Activation.Circulation. Cardiovascular imaging · 2018Review
- Molecular imaging of cardiac remodelling after myocardial infarction.Basic research in cardiology · 2018Review
- Functional and anatomical characterization of brown adipose tissue in heart failure with blood oxygen level dependent magnetic resonance.NMR in biomedicine · 2016Article
- Multimodality imaging demonstrates trafficking of liposomes preferentially to ischemic myocardium.Cardiovascular revascularization medicine : including molecular interventions · 2016Article
- Delayed contrast enhancement imaging of a murine model for ischemia reperfusion with carbon nanotube micro-CT.PloS one · 2015Article
- Molecular imaging of inflammation in atherosclerosis.Theranostics · 2013Review
- Imaging of early modification in cardiomyopathy: the doxorubicin-induced model.The international journal of cardiovascular imaging · 2013Review
- Multimodal iron oxide nanoparticles for hybrid biomedical imaging.NMR in biomedicine · 2013Review
- Fluorescence tomography of rapamycin-induced autophagy and cardioprotection in vivo.Circulation. Cardiovascular imaging · 2013Article
- Cardiac magnetic resonance imaging of rapid VCAM-1 up-regulation in myocardial ischemia-reperfusion injury.European biophysics journal : EBJ · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
backgroundA novel dual-contrast molecular MRI technique to image both cardiomyocyte apoptosis and necrosis in vivo within 4 to 6 hours of ischemia is presented. The technique uses the annexin-based nanoparticle AnxCLIO-Cy5.5 (apoptosis) and simultaneous delayed-enhancement imaging with a novel gadolinium chelate, Gd-DTPA-NBD (necrosis). METHODS AND
resultsMice with transient coronary ligation were injected intravenously at the onset of reperfusion with AnxCLIO-Cy5.5 (n=7) or the control probe Inact_CLIO-Cy5.5 (n=6). T2*-weighted MR images (9.4 T) were acquired within 4 to 6 hours of reperfusion. The contrast-to-noise ratio between injured and uninjured myocardium was measured. The mice were then injected with Gd-DTPA-NBD, and delayed-enhancement imaging was performed within 10 to 30 minutes. Uptake of AnxCLIO-Cy5.5 was most prominent in the midmyocardium and was significantly greater than that of Inact_CLIO-Cy5.5 (contrast-to-noise ratio, 8.82+/-1.5 versus 3.78+/-1.1; P<0.05). Only 21+/-3% of the myocardium with accumulation of AnxCLIO-Cy5.5 showed delayed-enhancement of Gd-DTPA-NBD. Wall thickening was significantly reduced in segments with delayed enhancement and/or transmural accumulation of AnxCLIO-Cy5.5 (P<0.001). Fluorescence microscopy of AnxCLIO-Cy5.5 and immunohistochemistry of Gd-DTPA-NBD confirmed the presence of large numbers of apoptotic but potentially viable cardiomyocytes (AnxCLIO-Cy5.5 positive, Gd-DTPA-NBD negative) in the midmyocardium.
conclusionsA novel technique to image cardiomyocyte apoptosis and necrosis in vivo within 4 to 6 hours of injury is presented and reveals large areas of apoptotic but viable myocardium in the midmyocardium. Strategies to salvage the numerous apoptotic but potentially viable cardiomyocytes in the midmyocardium in acute ischemia should be investigated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.