ArticleMolecular pain2009
Different forms of glycine- and GABA(A)-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neurons.
Article in Molecular pain, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Inhibitory Synaptic Influences on Developmental Motor Disorders.International journal of molecular sciences · 2023Review
- Synaptic Targets of Glycinergic Neurons in Laminae I-III of the Spinal Dorsal Horn.International journal of molecular sciences · 2023Article
- Kappa opioids inhibit the GABA/glycine terminals of rostral ventromedial medulla projections in the superficial dorsal horn of the spinal cord.The Journal of physiology · 2022Article
- GABACells · 2022Article
- Altered Intrinsic Properties and Inhibitory Connectivity in Aged Parvalbumin-Expressing Dorsal Horn Neurons.Frontiers in neural circuits · 2022Article
- Spinal microglial β-endorphin signaling mediates IL-10 and exenatide-induced inhibition of synaptic plasticity in neuropathic pain.CNS neuroscience & therapeutics · 2021Article
- Glycine Receptors in Spinal Nociceptive Control-An Update.Biomolecules · 2021Review
- Reviewing the case for compromised spinal inhibition in neuropathic pain.Journal of neural transmission (Vienna, Austria : 1996) · 2020Review
- PKCγ interneurons, a gateway to pathological pain in the dorsal horn.Journal of neural transmission (Vienna, Austria : 1996) · 2020Review
- Bmal1 knockdown suppresses wake and increases immobility without altering orexin A, corticotrophin-releasing hormone, or glutamate decarboxylase.CNS neuroscience & therapeutics · 2018Article
- Heteromeric α/β glycine receptors regulate excitability in parvalbumin-expressing dorsal horn neurons through phasic and tonic glycinergic inhibition.The Journal of physiology · 2017Article
- Distinct forms of synaptic inhibition and neuromodulation regulate calretinin-positive neuron excitability in the spinal cord dorsal horn.Neuroscience · 2016Article
- Infusion of Gabrα6 siRNA into the trigeminal ganglia increased the myogenic orofacial nociceptive response of ovariectomized rats treated with 17β-estradiol.Neuroscience · 2014Article
- Reduced GABAA receptor α6 expression in the trigeminal ganglion enhanced myofascial nociceptive response.Neuroscience · 2013Article
- Reduced GABA(A) receptor α6 expression in the trigeminal ganglion alters inflammatory TMJ hypersensitivity.Neuroscience · 2012Article
- Glycine receptor mutants of the mouse: what are possible routes of inhibitory compensation?Frontiers in molecular neuroscience · 2012Article
- Allosteric modulation of glycine receptors.British journal of pharmacology · 2011Review
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeurons in superficial (SDH) and deep (DDH) laminae of the spinal cord dorsal horn receive sensory information from skin, muscle, joints and viscera. In both regions, glycine- (GlyR) and GABAA-receptors (GABAARs) contribute to fast synaptic inhibition. For rat, several types of GABAAR coexist in the two regions and each receptor type provides different contributions to inhibitory tone. Recent work in mouse has discovered an additional type of GlyR, (containing alpha 3 subunits) in the SDH. The contribution of differing forms of the GlyR to sensory processing in SDH and DDH is not understood. METHODS AND
resultsHere we compare fast inhibitory synaptic transmission in mouse (P17-37) SDH and DDH using patch-clamp electrophysiology in transverse spinal cord slices (L3-L5 segments, 23 degrees C). GlyR-mediated mIPSCs were detected in 74% (25/34) and 94% (25/27) of SDH and DDH neurons, respectively. In contrast, GABAAR-mediated mIPSCs were detected in virtually all neurons in both regions (93%, 14/15 and 100%, 18/18). Several Gly- and GABAAR properties also differed in SDH vs. DDH. GlyR-mediated mIPSC amplitude was smaller (37.1 +/- 3.9 vs. 64.7 +/- 5.0 pA; n = 25 each), decay time was slower (8.5 +/- 0.8 vs. 5.5 +/- 0.3 ms), and frequency was lower (0.15 +/- 0.03 vs. 0.72 +/- 0.13 Hz) in SDH vs. DDH neurons. In contrast, GABAAR-mediated mIPSCs had similar amplitudes (25.6 +/- 2.4, n = 14 vs. 25. +/- 2.0 pA, n = 18) and frequencies (0.21 +/- 0.08 vs. 0.18 +/- 0.04 Hz) in both regions; however, decay times were slower (23.0 +/- 3.2 vs. 18.9 +/- 1.8 ms) in SDH neurons. Mean single channel conductance underlying mIPSCs was identical for GlyRs (54.3 +/- 1.6 pS, n = 11 vs. 55.7 +/- 1.8, n = 8) and GABAARs (22.7 +/- 1.7 pS, n = 10 vs. 22.4 +/- 2.0 pS, n = 11) in both regions. We also tested whether the synthetic endocanabinoid, methandamide (methAEA), had direct effects on Gly- and GABAARs in each spinal cord region. MethAEA (5 muM) reduced GlyR-mediated mIPSC frequency in SDH and DDH, but did not affect other properties. Similar results were observed for GABAAR mediated mIPSCs, however, rise time was slowed by methAEA in SDH neurons.
conclusionTogether these data show that Gly- and GABAARs with clearly differing physiological properties and cannabinoid-sensitivity contribute to fast synaptic inhibition in mouse SDH and DDH.
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