ArticleMolecular microbiology2010
The ATP-binding cassette transporter Cbc (choline/betaine/carnitine) recruits multiple substrate-binding proteins with strong specificity for distinct quaternary ammonium compounds.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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58 citing papers in PubMed, 94 citations in OpenAlex.
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- Bacterial catabolism of membrane phospholipids links marine biogeochemical cycles.Science advances · 2023Article
- Ubiquitous occurrence of a dimethylsulfoniopropionate ABC transporter in abundant marine bacteria.The ISME journal · 2023Article
- Prevalence and Antibiotic Resistance Phenotypes ofFoods (Basel, Switzerland) · 2023Article
- Novel functional insights into a modified sugar-binding protein from Synechococcus MITS9220.Scientific reports · 2022Article
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- In planta transcriptomics reveals conflicts between pattern-triggered immunity and the AlgU sigma factor regulon.PloS one · 2022Article
- Transcriptome Analysis to Understand Salt Stress Regulation Mechanism ofFrontiers in microbiology · 2022Article
- Bifidobacterium response to lactulose ingestion in the gut relies on a solute-binding protein-dependent ABC transporter.Communications biology · 2021Article
- Stressed out: Bacterial response to high salinity using compatible solute biosynthesis and uptake systems, lessons fromComputational and structural biotechnology journal · 2021Review
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5 authors at 2 institutions in 1 country.
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Abstract
We identified a choline, betaine and carnitine transporter, designated Cbc, from Pseudomonas syringae and Pseudomonas aeruginosa that is unusual among members of the ATP-binding cassette (ABC) transporter family in its use of multiple periplasmic substrate-binding proteins (SBPs) that are highly specific for their substrates. The SBP encoded by the cbcXWV operon, CbcX, binds choline with a high affinity (K(m), 2.6 microM) and, although it also binds betaine (K(m), 24.2 microM), CbcXWV-mediated betaine uptake did not occur in the presence of choline. The CbcX orthologue ChoX from Sinorhizobium meliloti was similar to CbcX in these binding properties. The core transporter CbcWV also interacts with the carnitine-specific SBP CaiX (K(m), 24 microM) and the betaine-specific SBP BetX (K(m), 0.6 microM). Unlike most ABC transporter loci, caiX, betX and cbcXWV are separated in the genome. CaiX-mediated carnitine uptake was reduced by CbcX and BetX only when they were bound by their individual ligands, providing the first in vivo evidence for a higher affinity for ligand-bound than ligand-free SBPs by an ABC transporter. These studies demonstrate not only that the Cbc transporter serves as a useful model for exploring ABC transporter component interactions, but also that the orphan SBP genes common to bacterial genomes can encode functional SBPs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.