ArticleJournal of the American Chemical Society2009
Femtosecond fluorescence spectra of tryptophan in human gamma-crystallin mutants: site-dependent ultrafast quenching.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 45 citations in OpenAlex.
- Mimicking oxidative damage in γS-crystallin with site-specific incorporation of 5-hydroxytryptophan.Biophysical reports · 2026Article
- Red edge excitation shift spectroscopy is highly sensitive to tryptophan composition.Journal of the Royal Society, Interface · 2023Article
- Ultrafast fluorescence dynamics of NADH in aprotic solvents: Quasi-static self-quenching unmasked.Journal of photochemistry and photobiology. A, Chemistry · 2023Article
- The mechanism for thermal-enhanced chaperone-like activity of α-crystallin against UV irradiation-induced aggregation of γD-crystallin.Biophysical journal · 2022Article
- Cataract-Causing S93R Mutant Destabilized Structural Conformation of βB1 Crystallin Linking With Aggregates Formation and Cellular Viability.Frontiers in molecular biosciences · 2022Article
- Ultrafast Fluorescence Spectroscopy via Upconversion and Its Applications in Biophysics.Molecules (Basel, Switzerland) · 2021Review
- Dehydrogenase Binding Sites Abolish the "Dark" Fraction of NADH: Implication for Metabolic Sensing via FLIM.The journal of physical chemistry. B · 2020Article
- Human αB-crystallin discriminates between aggregation-prone and function-preserving variants of a client protein.Biochimica et biophysica acta. General subjects · 2020Article
- Ultrafast Dynamics of Water-Protein Coupled Motions around the Surface of Eye Crystallin.Journal of the American Chemical Society · 2020Article
- A fraction of NADH in solution is "dark": Implications for metabolic sensing via fluorescence lifetime.Chemical physics letters · 2019Article
- Charge invariant protein-water relaxation in GB1 via ultrafast tryptophan fluorescence.Journal of the American Chemical Society · 2014Article
- Upconversion spectrophotofluorometry.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Amyloid fiber formation in human γD-Crystallin induced by UV-B photodamage.Biochemistry · 2013Article
- Femtosecond conical intersection dynamics of tryptophan in proteins and validation of slowdown of hydration layer dynamics.Journal of the American Chemical Society · 2012Article
- The molecular refractive function of lens γ-Crystallins.Journal of molecular biology · 2011Article
- Separating instability from aggregation propensity in γS-crystallin variants.Biophysical journal · 2011Article
- Tryptophan cluster protects human γD-crystallin from ultraviolet radiation-induced photoaggregation in vitro.Photochemistry and photobiologyArticle
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8 authors at 4 institutions in 1 country.
Funding
Abstract
The eye lens Crystallin proteins are subject to UV irradiation throughout life, and the photochemistry of damage proceeds through the excited state; thus, their tryptophan (Trp) fluorescence lifetimes are physiologically important properties. The time-resolved fluorescence spectra of single Trps in human gammaD- and gammaS-Crystallins have been measured with both an upconversion spectrophotofluorometer on the 300 fs to 100 ps time scale, and a time correlated single photon counting apparatus on the 100 ps to 10 ns time scale, respectively. Three Trps in each wild type protein were replaced by phenylalanine, leading to single-Trp mutants: W68-only and W156-only of HgammaD- and W72-only and W162-only of HgammaS-Crystallin. These proteins exhibit similar ultrafast signatures: positive definite decay associated spectra (DAS) for 50-65 ps decay constants that indicate dominance of fast, heterogeneous quenching. The quenched population (judged by amplitude) of this DAS differs among mutants. Trps 68, 156 in human gammaD- and Trp72 in human gammaS-Crystallin are buried, but water can reach amide oxygen and ring HE1 atoms through narrow channels. QM-MM simulations of quenching by electron transfer predict heterogeneous decay times from 50-500 ps that agree with our experimental results. Further analysis of apparent radiative lifetimes allow us to deduce that substantial subpopulations of Trp are fully quenched in even faster (sub-300 fs) processes for several of the mutants. The quenching of Trp fluorescence of human gammaD- and gammaS-Crystallin may protect them from ambient light induced photo damage.
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