ArticlePLoS biology2009
The multicopy gene Sly represses the sex chromosomes in the male mouse germline after meiosis.
Article in PLoS biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers.
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109 citing papers in PubMed, 174 citations in OpenAlex.
- The synaptonemal complex: structure, function, and clinical implications†.Biology of reproduction · 2026Article
- Testis-specific lncRNA Teshl regulates acrosome biogenesis to maintain sperm structure and function.Cell & bioscience · 2026Article
- Single cell RNA-sequencing reveals no evidence for meiotic sex chromosome inactivation in the threespine stickleback fish.PLoS genetics · 2025Article
- A guide to heat shock factors as multifunctional transcriptional regulators.The FEBS journal · 2025Review
- Single cell RNA-sequencing reveals no evidence for meiotic sex chromosome inactivation in the threespine stickleback fish.bioRxiv : the preprint server for biology · 2025Article
- The SHR/Akr Y chromosome reveals repeated turnover of the rat pseudoautosomal region.bioRxiv : the preprint server for biology · 2025Article
- Sex ratio drive in mice: A binding competition between sex-linked genes.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Reenacting a mouse genetic evolutionary arms race in yeast reveals that SLXL1/SLX compete with SLY1/2 for binding to Spindlins.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chromatin environment-dependent effects of DOT1L on gene expression in male germ cells.Communications biology · 2025Article
- Systematic identification of Y-chromosome gene functions in mouse spermatogenesis.Science (New York, N.Y.) · 2025Article
- The genetic mechanism of B chromosome drive in rye illuminated by chromosome-scale assembly.Nature communications · 2024Article
- Non-Mendelian transmission of X chromosomes: mechanisms and impact on sex ratios and population dynamics in different breeding systems.Biochemical Society transactions · 2024Review
- Recurrent Duplication and Diversification of a Vital DNA Repair Gene Family Across Drosophila.Molecular biology and evolution · 2024Article
- Genetic conflict and the origin of multigene families: implications for sex chromosome evolution.Proceedings. Biological sciences · 2023Review
- Return of the forgotten hero: the role of Y chromosome-encoded Zfy in male reproduction.Molecular human reproduction · 2023Article
- DOT1L regulates chromatin reorganization and gene expression during sperm differentiation.EMBO reports · 2023Article
- Bypassing Mendel's First Law: Transmission Ratio Distortion in Mammals.International journal of molecular sciences · 2023Review
- The contribution of sex chromosome conflict to disrupted spermatogenesis in hybrid house mice.Genetics · 2022Article
- Gonadal mosaicism mediated female-biased gender control in mice.Protein & cell · 2022Article
- Two acquired mouse Y chromosome-linked genes, Prssly and Teyorf1, are dispensable for male fertility‡.Biology of reproduction · 2022Article
49 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Studies of mice with Y chromosome long arm deficiencies suggest that the male-specific region (MSYq) encodes information required for sperm differentiation and postmeiotic sex chromatin repression (PSCR). Several genes have been identified on MSYq, but because they are present in more than 40 copies each, their functions cannot be investigated using traditional gene targeting. Here, we generate transgenic mice producing small interfering RNAs that specifically target the transcripts of the MSYq-encoded multicopy gene Sly (Sycp3-like Y-linked). Microarray analyses performed on these Sly-deficient males and on MSYq-deficient males show a remarkable up-regulation of sex chromosome genes in spermatids. SLY protein colocalizes with the X and Y chromatin in spermatids of normal males, and Sly deficiency leads to defective repressive marks on the sex chromatin, such as reduced levels of the heterochromatin protein CBX1 and of histone H3 methylated at lysine 9. Sly-deficient mice, just like MSYq-deficient mice, have severe impairment of sperm differentiation and are near sterile. We propose that their spermiogenesis phenotype is a consequence of the change in spermatid gene expression following Sly deficiency. To our knowledge, this is the first successful targeted disruption of the function of a multicopy gene (or of any Y gene). It shows that SLY has a predominant role in PSCR, either via direct interaction with the spermatid sex chromatin or via interaction with sex chromatin protein partners. Sly deficiency is the major underlying cause of the spectrum of anomalies identified 17 y ago in MSYq-deficient males. Our results also suggest that the expansion of sex-linked spermatid-expressed genes in mouse is a consequence of the enhancement of PSCR that accompanies Sly amplification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.