ArticleThe Journal of cell biology1991
Modulation of hexokinase association with mitochondria analyzed with quantitative three-dimensional confocal microscopy.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 83 citations in OpenAlex.
- Glycolysis and glycolytic enzymes in acute myeloid leukemia: Warburg and beyond.Frontiers in pharmacology · 2026Review
- Mechanistic multiscale modelling of energy metabolism in human astrocytes reveals the impact of morphology changes in Alzheimer's Disease.PLoS computational biology · 2023Article
- Bioenergetic adaptations to HIV infection. Could modulation of energy substrate utilization improve brain health in people living with HIV-1?Experimental neurology · 2020Review
- Article
- Metabolism of Mannose in Cultured Primary Rat Neurons.Neurochemical research · 2017Article
- Reducing mitochondrial bound hexokinase II mediates transition from non-injurious into injurious ischemia/reperfusion of the intact heart.Journal of physiology and biochemistry · 2016Article
- Post-translational Regulation of Hexokinase Function and Protein Stability in the Aestivating Frog Xenopus laevis.The protein journal · 2016Article
- Fatty acids in energy metabolism of the central nervous system.BioMed research international · 2014Review
- Diffusion of D-glucose measured in the cytosol of a single astrocyte.Cellular and molecular life sciences : CMLS · 2013Article
- Pathophysiological consequences of TAT-HKII peptide administration are independent of impaired vascular function and ensuing ischemia.Circulation research · 2013Article
- Article
- Anticancer agents that counteract tumor glycolysis.ChemMedChem · 2012Review
- Aspects of astrocyte energy metabolism, amino acid neurotransmitter homoeostasis and metabolic compartmentation.ASN neuro · 2012Review
- Glial cells in (patho)physiology.Journal of neurochemistry · 2012Review
- A practical guide to evaluating colocalization in biological microscopy.American journal of physiology. Cell physiology · 2011Article
- Mannose efflux from the cells: a potential source of mannose in blood.The Journal of biological chemistry · 2011Article
- Emerging metabolic targets in cancer therapy.Frontiers in bioscience (Landmark edition) · 2011Review
- Multi-image colocalization and its statistical significance.Biophysical journal · 2010Article
- Identification of pancreatic cancer-specific cell-surface markers for development of targeting ligands.Methods in molecular biology (Clifton, N.J.) · 2010Article
- Enhanced targeting with heterobivalent ligands.Molecular cancer therapeutics · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Hexokinase isozyme I is proposed to be associated with mitochondria in vivo. Moreover, it has been suggested that this association is modulated in coordination with changes in cell metabolic state. To test these hypotheses, we analyzed the subcellular distribution of hexokinase relative to mitochondria in paraformaldehyde-fixed astrocytes using immunocytochemistry and quantitative three-dimensional confocal microscopy. Analysis of the extent of colocalization between hexokinase and mitochondria revealed that approximately 70% of cellular hexokinase is associated with mitochondria under basal metabolic conditions. In contrast to the immunocytochemical studies, between 15 to 40% of cellular hexokinase was found to be associated with mitochondria after fractionation of astrocyte cultures depending on the exact fractionation conditions. The discrepancy between fractionation studies and those based on imaging of distributions in fixed cells indicates the usefulness of using techniques that can evaluate the distributions of "cytosolic" enzymes in cells whose subcellular ultrastructure is not severely disrupted. To determine if hexokinase distribution is modulated in concert with changes in cell metabolism, the localization of hexokinase with mitochondria was evaluated after inhibition of glucose metabolism with 2-deoxyglucose. After incubation with 2-deoxyglucose there was an approximate 35% decrease in the amount of hexokinase associated with mitochondria. These findings support the hypothesis that hexokinase is bound to mitochondria in rat brain astrocytes in vivo, and that this association is sensitive to cell metabolic state.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.