ArticleInfection and immunity2010
Aspergillus fumigatus LaeA-mediated phagocytosis is associated with a decreased hydrophobin layer.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- MAPK Signaling Pathway May Directly Regulate the Expression of Hydrophobin Genes inJournal of fungi (Basel, Switzerland) · 2026Article
- The global regulator LaeA controls expression of genes involved in diverse primary and secondary metabolites biosynthesis and their secretion in fungi.Frontiers in microbiology · 2026Review
- Preparation and Characterization of Hydrophobin 4-Coated Liposomes for Doxorubicin Delivery to Cancer Cells.Pharmaceuticals (Basel, Switzerland) · 2024Article
- Beyond morphogenesis and secondary metabolism: function of Velvet proteins and LaeA in fungal pathogenesis.Applied and environmental microbiology · 2024Review
- Card9 and MyD88 differentially regulate Th17 immunity to the commensal yeastbioRxiv : the preprint server for biology · 2024Article
- Categorizing Sequences of Concern by Function To Better Assess Mechanisms of Microbial Pathogenesis.Infection and immunity · 2022Review
- Isoeugenol affects expression pattern of conidial hydrophobin gene RodA and transcriptional regulators MedA and SomA responsible for adherence and biofilm formation in Aspergillus fumigatus.Archives of microbiology · 2022Article
- A bacterial endosymbiont of the fungus Rhizopus microsporus drives phagocyte evasion and opportunistic virulence.Current biology : CB · 2022Article
- The BcLAE1 is involved in the regulation of ABA biosynthesis inFrontiers in microbiology · 2022Article
- Fungal immunity and pathogenesis in mammals versus the invertebrate model organism Galleria mellonella.Pathogens and disease · 2021Review
- Aspergillus fumigatus Cell Wall Promotes Apical Airway Epithelial Recruitment of Human Neutrophils.Infection and immunity · 2020Article
- Assembly and disassembly ofCell surface (Amsterdam, Netherlands) · 2019Article
- Transcriptomic Insights into Benzenamine Effects on the Development, Aflatoxin Biosynthesis, and Virulence ofToxins · 2019Article
- Microbial volatile communication in human organotypic lung models.Nature communications · 2017Article
- Characterization of C-type lectins reveals an unexpectedly limited interaction between Cryptococcus neoformans spores and Dectin-1.PloS one · 2017Article
- Interactions of fungal pathogens with phagocytes.Nature reviews. Microbiology · 2016Review
- Genome-wide gene expression dynamics of the fungal pathogen Dothistroma septosporum throughout its infection cycle of the gymnosperm host Pinus radiata.Molecular plant pathology · 2016Article
- Challenges and Strategies for Proteome Analysis of the Interaction of Human Pathogenic Fungi with Host Immune Cells.Proteomes · 2015Review
- The Aspergillus fumigatus cell wall integrity signaling pathway: drug target, compensatory pathways, and virulence.Frontiers in microbiology · 2015Review
- Distinct innate immune phagocyte responses to Aspergillus fumigatus conidia and hyphae in zebrafish larvae.Eukaryotic cell · 2014Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Aspergillus fumigatus is the causal agent of the life-threatening disease invasive aspergillosis. A. fumigatus laeA deletants, aberrant in toxin biosynthesis and spore development, are decreased in virulence. Among other characteristics, the decreased virulence is associated with increased spore susceptibility to macrophage phagocytosis. Three characteristics, cell wall microbe-associated molecular patterns (MAMPs), secreted metabolites, and rodlet content, thought to be important in macrophage-Aspergillus spore interactions were examined. Flow cytometry analysis of wild-type and DeltalaeA spores did not reveal any differences in surface-accessible MAMPs, including beta-(1,3)-glucan, alpha-mannose, chitin, and other carbohydrate ligands. Blocking experiments with laminarin and mannan supported the conclusion that differences in cell wall carbohydrates were not responsible for enhanced DeltalaeA spore phagocytosis. Aspergillus spores have been reported to secrete metabolites affecting phagocytosis. Neither spent culture exchange, transwell, nor coincubation internalization experiments supported a role for secreted metabolites in the differential uptake of wild-type and DeltalaeA spores. However, sonication assays implicated a role for surface rodlet protein/hydrophobin (RodAp) in differential spore phagocytosis. A possible role of RodAp in enhanced DeltalaeA spore uptake was further assessed by RodAp extraction and quantification, where wild-type spores were found to contain 60% more RodAp than DeltalaeA spores. After removal of the surface rodlet layer, wild-type spores were phagocytosed at similar rates as DeltalaeA spores. We conclude that increased uptake of DeltalaeA resting spores is not associated with changes in secreted metabolite production of this mutant or surface carbohydrate availability but, rather, due to a decrease in the surface RodAp content of DeltalaeA spores. We theorize that RodAp acts as an antiphagocytic molecule, possibly via physicochemical means and/or by impeding MAMP recognition by macrophage receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.