ArticleThe Journal of biological chemistry2010
Integrin alphaIIbbeta3 activation in Chinese hamster ovary cells and platelets increases clustering rather than affinity.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 55 citations in OpenAlex.
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- PPM1F controls integrin activity via a conserved phospho-switch.The Journal of cell biology · 2020Article
- The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.Journal of cell science · 2020Article
- Multiscale model of integrin adhesion assembly.PLoS computational biology · 2019Article
- Microfluidic auto-alignment of protein patterns for dissecting multi-receptor crosstalk in platelets.Lab on a chip · 2018Article
- Conformational Equilibrium of Human Platelet Integrin Investigated by Three-Dimensional Electron Cryo-Microscopy.Sub-cellular biochemistry · 2018Review
- Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.Journal of thrombosis and haemostasis : JTH · 2017Article
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- The talin head domain reinforces integrin-mediated adhesion by promoting adhesion complex stability and clustering.PLoS genetics · 2014Article
- Integrin-associated complexes form hierarchically with variable stoichiometry in nascent adhesions.Current biology : CB · 2014Article
- Selective targeting of TGF-β activation to treat fibroinflammatory airway disease.Science translational medicine · 2014Article
- Structural and mechanical functions of integrins.Biophysical reviews · 2014Review
- Talin-bound NPLY motif recruits integrin-signaling adapters to regulate cell spreading and mechanosensing.The Journal of cell biology · 2014Article
- Mechanisms of talin-dependent integrin signaling and crosstalk.Biochimica et biophysica acta · 2014Review
- The mechanism of kindlin-mediated activation of integrin αIIbβ3.Current biology : CB · 2013Article
- Talins and kindlins: partners in integrin-mediated adhesion.Nature reviews. Molecular cell biology · 2013Review
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Integrin alphaIIbbeta3 affinity regulation by talin binding to the cytoplasmic tail of beta3 is a generally accepted model for explaining activation of this integrin in Chinese hamster ovary cells and human platelets. Most of the evidence for this model comes from the use of multivalent ligands. This raises the possibility that the activation being measured is that of increased clustering of the integrin rather than affinity. Using a newly developed assay that probes integrins on the surface of cells with only monovalent ligands prior to fixation, I do not find increases in affinity of alphaIIbbeta3 integrins by talin head fragments in Chinese hamster ovary cells, nor do I observe affinity increases in human platelets stimulated with thrombin. Binding to a multivalent ligand does increase in both of these cases. This assay does report affinity increases induced by either Mn(2+), a cytoplasmic domain mutant (D723R) in the cytoplasmic domain of beta3, or preincubation with a peptide ligand. These results reconcile the previously observed differences between talin effects on integrin activation in Drosophila and vertebrate systems and suggest new models for talin regulation of integrin activity in human platelets.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.