ArticleGastroenterology2010
A model to study the phenotypic changes of interstitial cells of Cajal in gastrointestinal diseases.
Article in Gastroenterology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 70 citations in OpenAlex.
- Changes in interstitial cells of Cajal calcium activity in a diabetic mouse model of delayed gastric emptying.The Journal of physiology · 2026Article
- Sustained Effectiveness and Safety of Therapeutic miR-10a/b in Alleviating Diabetes and Gastrointestinal Dysmotility without Inducing Cancer or Inflammation in Murine Liver and Colon.International journal of molecular sciences · 2024Article
- CaPhysiological reviews · 2024Review
- Comparison of laxative effects of fermented soybeans (Nutrition research and practice · 2022Article
- CaThe Journal of physiology · 2022Article
- CaAdvances in experimental medicine and biology · 2022Article
- Dnmt2-null sperm block maternal transmission of a paramutant phenotype†.Biology of reproduction · 2021Article
- Colonic Motility Is Improved by the Activation of 5-HTGastroenterology · 2021Article
- miR-10b-5p Rescues Diabetes and Gastrointestinal Dysmotility.Gastroenterology · 2021Article
- CaeLife · 2021Article
- Excitatory cholinergic responses in mouse colon intramuscular interstitial cells of Cajal are due to enhanced CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Identification and classification of interstitial cells in the mouse renal pelvis.The Journal of physiology · 2020Article
- Understanding the Biology of Human Interstitial Cells of Cajal in Gastrointestinal Motility.International journal of molecular sciences · 2020Review
- Investigation of Novel c-Kit-expressing Smooth Muscle Cells in Murine Cecum.Acta histochemica et cytochemica · 2020Article
- CaThe Journal of physiology · 2019Article
- Smooth Muscle Transcriptome Browser: offering genome-wide references and expression profiles of transcripts expressed in intestinal SMC, ICC, and PDGFRαScientific reports · 2019Article
- Spontaneous Electrical Activity and Rhythmicity in Gastrointestinal Smooth Muscles.Advances in experimental medicine and biology · 2019Review
- The effects of mitochondrial inhibitors on CaCell calcium · 2018Article
- DNA methylation, through DNMT1, has an essential role in the development of gastrointestinal smooth muscle cells and disease.Cell death & disease · 2018Article
- The Potential for Gut Organoid Derived Interstitial Cells of Cajal in Replacement Therapy.International journal of molecular sciences · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsInterstitial cells of Cajal (ICC) express the receptor tyrosine kinase, KIT, the receptor for stem cell factor. In the gastrointestinal (GI) tract, ICC are pacemaker cells that generate spontaneous electrical slow waves, and mediate inputs from motor neurons. Absence or loss of ICC are associated with GI motility disorders, including those consequent of diabetes. Studies of ICC have been hampered by the low density of these cells and difficulties in recognizing these cells in cell dispersions.
methodsKit(+/copGFP) mice harboring a copepod super green fluorescent protein (copGFP) complementary DNA, inserted at the Kit locus, were generated. copGFP(+) ICC from GI muscles were analyzed using confocal microscopy and flow cytometry. copGFP(+) ICC from the jejunum were purified by a fluorescence-activated cell sorter and validated by cell-specific markers. Kit(+/copGFP) mice were crossbred with diabetic Lep(+/ob) mice to generate compound Kit(+/copGFP);Lep(ob/ob) mutant mice. copGFP(+) ICC from compound transgenic mice were analyzed by confocal microscopy.
resultscopGFP in Kit(+/copGFP) mice colocalized with KIT immunofluorescence and thus was predominantly found in ICC. In other smooth muscles, mast cells were also labeled, but these cells were relatively rare in the murine GI tract. copGFP(+) cells from jejunal muscles were Kit(+) and free of contaminating cell-specific markers. Kit(+/copGFP);Lep(ob/ob) mice displayed ICC networks that were dramatically disrupted during the development of diabetes.
conclusionsKit(+/copGFP) mice offer a powerful new model to study the function and genetic regulation of ICC phenotypes. Isolation of ICC from animal models will help determine the causes and responses of ICC to therapeutic agents.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.