ArticleMolecular cell2009
A reciprocal interdependence between Nck and PI(4,5)P(2) promotes localized N-WASp-mediated actin polymerization in living cells.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- The class VIII myosin ATM2 stabilizes actin-plasma membrane contacts in tobacco pollen tubes by locally promoting PtdIns(4,5)PNature plants · 2026Article
- Implicates of PIP5K1α in asthma-related biological processes: insights into mechanisms and therapeutic potential.Journal of translational medicine · 2025Review
- De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells.Nature communications · 2024Article
- Nck adaptors at a glance.Journal of cell science · 2021Article
- A Membrane-Tethered Ubiquitination Pathway Regulates Hedgehog Signaling and Heart Development.Developmental cell · 2020Article
- Computer simulations of protein-membrane systems.Progress in molecular biology and translational science · 2020Article
- Nck adapter proteins promote podosome biogenesis facilitating extracellular matrix degradation and cancer invasion.Cancer medicine · 2019Article
- Nck deficiency is associated with delayed breast carcinoma progression and reduced metastasis.Molecular biology of the cell · 2017Article
- Mechanistic principles underlying regulation of the actin cytoskeleton by phosphoinositides.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cytoskeletal control of B cell responses to antigens.Nature reviews. Immunology · 2017Review
- Article
- Integration of linear and dendritic actin nucleation in Nck-induced actin comets.Molecular biology of the cell · 2016Article
- n-3 polyunsaturated fatty acids suppress CD4(+) T cell proliferation by altering phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2] organization.Biochimica et biophysica acta · 2016Article
- The multifaceted role of PIP2 in leukocyte biology.Cellular and molecular life sciences : CMLS · 2015Review
- The Nck-interacting kinase NIK increases Arp2/3 complex activity by phosphorylating the Arp2 subunit.The Journal of cell biology · 2015Article
- Phosphatase of regenerating liver 3 (PRL3) provokes a tyrosine phosphoproteome to drive prometastatic signal transduction.Molecular & cellular proteomics : MCP · 2013Article
- Phosphatidylinositol 4,5-bisphosphate: targeted production and signaling.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Review
- Low hippocampal PI(4,5)P₂ contributes to reduced cognition in old mice as a result of loss of MARCKS.Nature neuroscience · 2013Article
- Phosphatidic acid regulation of PIPKI is critical for actin cytoskeletal reorganization.Journal of lipid research · 2012Article
- Stoichiometry of Nck-dependent actin polymerization in living cells.The Journal of cell biology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Modulation of actin dynamics through the N-WASp/Arp2/3 pathway is important in cell locomotion, membrane trafficking, and pathogen infection. Here, we demonstrate that Nck is essential for actin remodeling stimulated by phosphatidylinositol 4,5 bisphosphate (PI(4,5)P(2)) and, conversely, that PI(4,5)P(2) is necessary for localized actin polymerization induced by Nck in vivo. Nck knockdown or knockout suppressed actin comets induced by phosphatidylinositol 5-kinase (PIP5K), and PIP5K stimulated tyrosine phosphorylation of an Nck SH2 domain binding partner, suggesting that Nck couples phosphotyrosine- and phosphoinositide-dependent signals. We show that PI(4,5)P(2) and PIP5K are both enriched at actin comets induced by Nck aggregates and that formation of actin comets was strongly inhibited by coclustering with an inositol 5-phosphatase domain to decrease local PI(4,5)P(2) levels. The extent of Nck-induced actin polymerization was also modulated by PI(4,5)P(2)-sensitive N-WASp mutants. This study uncovers a strong reciprocal interdependence between Nck and PI(4,5)P(2) in promoting localized N-WASp-mediated actin polymerization in cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.