ArticleBiophysical journal2009
Quantitative imaging of molecular order in lipid membranes using two-photon fluorescence polarimetry.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.Nature communications · 2026Article
- Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy.bioRxiv : the preprint server for biology · 2025Article
- Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?Membranes · 2025Review
- Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.Journal of cell science · 2024Review
- OOPS: Object-Oriented Polarization Software for analysis of fluorescence polarization microscopy images.PLoS computational biology · 2024Article
- Dynamic Mode Decomposition of Multiphoton and Stimulated Emission Depletion Microscopy Data for Analysis of Fluorescent Probes in Cellular Membranes.Sensors (Basel, Switzerland) · 2024Article
- Orthogonally-polarized excitation for improved two-photon and second-harmonic-generation microscopy, applied to neurotransmitter imaging with GPCR-based sensors.Biomedical optics express · 2022Article
- Linearly polarized excitation enhances signals from fluorescent voltage indicators.Biophysical journal · 2021Article
- High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes.Nature communications · 2020Article
- Influence of Membrane Phase on the Optical Properties of DPH.Molecules (Basel, Switzerland) · 2020Article
- Effects of excitation light polarization on fluorescence emission in two-photon light-sheet microscopy.Biomedical optics express · 2020Article
- Advances of super-resolution fluorescence polarization microscopy and its applications in life sciences.Computational and structural biotechnology journal · 2020Review
- Super-resolution dipole orientation mapping via polarization demodulation.Light, science & applications · 2016Article
- Molecular organization, localization and orientation of antifungal antibiotic amphotericin B in a single lipid bilayer.Scientific reports · 2016Article
- Differences in the location of guest molecules within zeolite pores as revealed by multilaser excitation confocal fluorescence microscopy: which molecule is where?Journal of the American Chemical Society · 2015Article
- Photobleaching kinetics and time-integrated emission of fluorescent probes in cellular membranes.Molecules (Basel, Switzerland) · 2014Article
- Ultimate use of two-photon fluorescence microscopy to map orientational behavior of fluorophores.Biophysical journal · 2014Article
- The role of molecular dipole orientation in single-molecule fluorescence microscopy and implications for super-resolution imaging.Chemphyschem : a European journal of chemical physics and physical chemistry · 2014Review
- Polarization-controlled TIRFM with focal drift and spatial field intensity correction.Biophysical journal · 2014Article
- Rotational mobility of single molecules affects localization accuracy in super-resolution fluorescence microscopy.Nano letters · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a polarimetric two-photon microscopy technique to quantitatively image the local static molecular orientational behavior in lipid and cell membranes. This approach, based on a tunable excitation polarization state complemented by a polarized readout, is easily implementable and does not require hypotheses on the molecular angular distribution such as its mean orientation, which is a main limitation in traditional fluorescence anisotropy measurements. The method is applied to the investigation of the molecular angular distribution in giant unilamellar vesicles formed by liquid-ordered and liquid-disordered micro-domains, and in COS-7 cell membranes. The highest order contrast between ordered and disordered domains is obtained for dyes locating within the membrane acyl chains.
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