ArticleBiophysical journal2009
Configuration of PKCalpha-C2 domain bound to mixed SOPC/SOPS lipid monolayers.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- CaThe journal of physical chemistry. B · 2025Article
- Uncovering Membrane-Bound Models of Coagulation Factors by Combined Experimental and Computational Approaches.Thrombosis and haemostasis · 2021Review
- Sensitivity of peripheral membrane proteins to the membrane context: A case study of phosphatidylserine and the TIM proteins.Biochimica et biophysica acta. Biomembranes · 2018Review
- Coupling X-Ray Reflectivity and In Silico Binding to Yield Dynamics of Membrane Recognition by Tim1.Biophysical journal · 2017Article
- Capturing Spontaneous Membrane Insertion of the Influenza Virus Hemagglutinin Fusion Peptide.The journal of physical chemistry. B · 2015Article
- Zooming in on disordered systems: neutron reflection studies of proteins associated with fluid membranes.Biochimica et biophysica acta · 2014Review
- Molecular mechanism for differential recognition of membrane phosphatidylserine by the immune regulatory receptor Tim4.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Single-molecule studies reveal a hidden key step in the activation mechanism of membrane-bound protein kinase C-α.Biochemistry · 2014Article
- Classical protein kinases C are regulated by concerted interaction with lipids: the importance of phosphatidylinositol-4,5-bisphosphate.Biophysical reviews · 2014Review
- Conformational transition of membrane-associated terminally acylated HIV-1 Nef.Structure (London, England : 1993) · 2013Article
- Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment.Nature structural & molecular biology · 2013Article
- Correlation between composition of the outer layer and phase asymmetry for vesicles ruptured by phospholipase D.The Journal of membrane biology · 2013Article
- Effect of mixed-phospholipid layer on phospholipase D reaction-induced vesicle rupture.The Journal of membrane biology · 2012Article
- Effect of phospholipid bilayer phase asymmetry on phospholipase d reaction-induced vesicle rupture.The Journal of membrane biology · 2011Article
- Electrostatic interactions and binding orientation of HIV-1 matrix studied by neutron reflectivity.Biophysical journal · 2010Article
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Authors and funding
7 authors.
Funding
Abstract
X-ray reflectivity measurements are used to determine the configuration of the C2 domain of protein kinase Calpha (PKCalpha-C2) bound to a lipid monolayer of a 7:3 mixture of 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine and 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine supported on a buffered aqueous solution. The reflectivity is analyzed in terms of the known crystallographic structure of PKCalpha-C2 and a slab model representation of the lipid layer. The configuration of lipid-bound PKCalpha-C2 is described by two angles that define its orientation, theta = 35 degrees +/- 10 degrees and phi =210 degrees +/- 30 degrees, and a penetration depth (=7.5 +/- 2 A) into the lipid layer. In this structure, the beta-sheets of PKCalpha-C2 are nearly perpendicular to the lipid layer and the domain penetrates into the headgroup region of the lipid layer, but not into the tailgroup region. This configuration of PKCalpha-C2 determined by our x-ray reflectivity is consistent with many previous findings, particularly mutational studies, and also provides what we believe is new molecular insight into the mechanism of PKCalpha enzyme activation. Our analysis method, which allows us to test all possible protein orientations, shows that our data cannot be explained by a protein that is orientated parallel to the membrane, as suggested by earlier work.
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