ArticleBMC genomics2009
Analysis of expressed sequence tags and identification of genes encoding cell-wall-degrading enzymes from the fungivorous nematode Aphelenchus avenae.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 40 citations in OpenAlex.
- Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode.Mycorrhiza · 2022Article
- AFrontiers in plant science · 2021Article
- Fungi-Nematode Interactions: Diversity, Ecology, and Biocontrol Prospects in Agriculture.Journal of fungi (Basel, Switzerland) · 2020Review
- Detoxification-related gene expression accompanies anhydrobiosis in the foliar nematode (Journal of nematology · 2020Article
- Assessment of the behaviour and survival of nematodes under low oxygen concentrations.PloS one · 2018Article
- Genomics and Comparative Genomic Analyses Provide Insight into the Taxonomy and Pathogenic Potential of NovelFrontiers in cellular and infection microbiology · 2017Article
- Horizontal gene transfer of acetyltransferases, invertases and chorismate mutases from different bacteria to diverse recipients.BMC evolutionary biology · 2016Article
- Novel Pectate Lyase Genes of Heterodera glycines Play Key Roles in the Early Stage of Parasitism.PloS one · 2016Article
- Distribution and evolution of glycoside hydrolase family 45 cellulases in nematodes and fungi.BMC evolutionary biology · 2014Article
- Transcriptomic analysis of the rice white tip nematode, Aphelenchoides besseyi (Nematoda: Aphelenchoididae).PloS one · 2014Article
- Exploring the host parasitism of the migratory plant-parasitic nematode Ditylenchus destuctor by expressed sequence tags analysis.PloS one · 2013Article
- Rather than by direct acquisition via lateral gene transfer, GHF5 cellulases were passed on from early Pratylenchidae to root-knot and cyst nematodes.BMC evolutionary biology · 2012Article
- A molecular analysis of desiccation tolerance mechanisms in the anhydrobiotic nematode Panagrolaimus superbus using expressed sequenced tags.BMC research notes · 2012Article
- Insights into metazoan evolution from Alvinella pompejana cDNAs.BMC genomics · 2010Article
- Multiple lateral gene transfers and duplications have promoted plant parasitism ability in nematodes.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fungivorus nematode, Aphelenchus avenae is widespread in soil and is found in association with decaying plant material. This nematode is also found in association with plants but its ability to cause plant disease remains largely undetermined. The taxonomic position and intermediate lifestyle of A. avenae make it an important model for studying the evolution of plant parasitism within the Nematoda. In addition, the exceptional capacity of this nematode to survive desiccation makes it an important system for study of anhydrobiosis. Expressed sequence tag (EST) analysis may therefore be useful in providing an initial insight into the poorly understood genetic background of A. avenae.
resultsWe present the generation, analysis and annotation of over 5,000 ESTs from a mixed-stage A. avenae cDNA library. Clustering of 5,076 high-quality ESTs resulted in a set of 2,700 non-redundant sequences comprising 695 contigs and 2,005 singletons. Comparative analyses indicated that 1,567 (58.0%) of the cluster sequences had homologues in Caenorhabditis elegans, 1,750 (64.8%) in other nematodes, 1,321(48.9%) in organisms other than nematodes, and 862 (31.9%) had no significant match to any sequence in current protein or nucleotide databases. In addition, 1,100 (40.7%) of the sequences were functionally classified using Gene Ontology (GO) hierarchy. Similarity searches of the cluster sequences identified a set of genes with significant homology to genes encoding enzymes that degrade plant or fungal cell walls. The full length sequences of two genes encoding glycosyl hydrolase family 5 (GHF5) cellulases and two pectate lyase genes encoding polysaccharide lyase family 3 (PL3) proteins were identified and characterized.
conclusionWe have described at least 2,214 putative genes from A. avenae and identified a set of genes encoding a range of cell-wall-degrading enzymes. This EST dataset represents a starting point for studies in a number of different fundamental and applied areas. The presence of genes encoding a battery of cell-wall-degrading enzymes in A. avenae and their similarities with genes from other plant parasitic nematodes suggest that this nematode can act not only as a fungal feeder but also a plant parasite. Further studies on genes encoding cell-wall-degrading enzymes in A. avenae will accelerate our understanding of the complex evolutionary histories of plant parasitism and the use of genes obtained by horizontal gene transfer from prokaryotes.
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