ArticleMicrobial drug resistance (Larchmont, N.Y.)2010
Development of a DNA microarray to detect antimicrobial resistance genes identified in the National Center for Biotechnology Information database.
Article in Microbial drug resistance (Larchmont, N.Y.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review.Molecules (Basel, Switzerland) · 2019Pooled it
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- The Requirement of Genetic Diagnostic Technologies for Environmental Surveillance of Antimicrobial Resistance.Sensors (Basel, Switzerland) · 2021Article
- Rapid and real-time monitoring of bacterial growth against antibiotics in solid growth medium using a contactless planar microwave resonator sensor.Scientific reports · 2021Article
- Rapid Antimicrobial Susceptibility Testing on Clinical Urine Samples by Video-Based Object Scattering Intensity Detection.Analytical chemistry · 2021Article
- Nanomotion Detection-Based Rapid Antibiotic Susceptibility Testing.Antibiotics (Basel, Switzerland) · 2021Review
- Direct Antimicrobial Susceptibility Testing on Clinical Urine Samples by Optical Tracking of Single Cell Division Events.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Current state of the art in rapid diagnostics for antimicrobial resistance.Lab on a chip · 2020Review
- Current and Emerging Methods of Antibiotic Susceptibility Testing.Diagnostics (Basel, Switzerland) · 2019Review
- Gut Bacterial Microbiota and its Resistome Rapidly Recover to Basal State Levels after Short-term Amoxicillin-Clavulanic Acid Treatment in Healthy Adults.Scientific reports · 2018Article
- Systematic review in South Africa reveals antibiotic resistance genes shared between clinical and environmental settings.Infection and drug resistance · 2018Review
- National Antimicrobial Resistance Monitoring System: Two Decades of Advancing Public Health Through Integrated Surveillance of Antimicrobial Resistance.Foodborne pathogens and disease · 2017Review
- Modeling Hybridization Kinetics of Gene Probes in a DNA Biochip Using FEMLAB.Microarrays (Basel, Switzerland) · 2017Article
- Whole Genome Sequencing for Surveillance of Antimicrobial Resistance inFrontiers in microbiology · 2017Article
- Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards.Applied microbiology and biotechnology · 2015Article
- Antimicrobial resistance genes in multidrug-resistant Salmonella enterica isolated from animals, retail meats, and humans in the United States and Canada.Microbial drug resistance (Larchmont, N.Y.) · 2013Article
- Molecular characterization of multidrug resistant hospital isolates using the antimicrobial resistance determinant microarray.PloS one · 2013Article
- Article
- Microarray-based analysis of IncA/C plasmid-associated genes from multidrug-resistant Salmonella enterica.Applied and environmental microbiology · 2011Article
- Development of a DNA microarray for enterococcal species, virulence, and antibiotic resistance gene determinations among isolates from poultry.Applied and environmental microbiology · 2011Article
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
Abstract
To understand the mechanisms and epidemiology of antimicrobial resistance (AR), the genetic elements responsible must be identified. Due to the myriad of possible genes, a high-density genotyping technique is needed for initial screening. To achieve this, AR genes in the National Center for Biotechnology Information GenBank database were identified by their annotations and compiled into a nonredundant list of 775 genes. A DNA microarray was constructed of 70mer oligonucelotide probes designed to detect these genes encoding resistances to aminoglycosides, beta-lactams, chloramphenicols, glycopeptides, heavy metals, lincosamides, macrolides, metronidazoles, polyketides, quaternary ammonium compounds, streptogramins, sulfonamides, tetracyclines, and trimethoprims as well as resistance transfer genes. The microarray was validated with two fully sequenced control strains of Salmonella enterica: Typhimurium LT2 (sensitive) and Typhi CT18 (multidrug resistance [MDR]). All resistance genes encoded on the MDR plasmid, pHCM1, harbored by CT18 were detected in that strain, whereas no resistance genes were detected in LT2. The microarray was also tested with a variety of bacteria, including MDR Salmonella enterica serovars, Escherichia coli, Campylobacter spp., Enterococcus spp., methicillin-resistant Staphylococcus aureus, Listeria spp., and Clostridium difficile. The results presented here demonstrate that a microarray can be designed to detect virtually all AR genes found in the National Center for Biotechnology Information database, thus reducing the subsequent assays necessary to identify specific resistance gene alleles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.