ArticleIn vitro cellular & developmental biology. Animal2010
Human colon tissue in organ culture: preservation of normal and neoplastic characteristics.
Article in In vitro cellular & developmental biology. Animal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Development of an Oral Epithelial Ex Vivo Organ Culture Model for Biocompatibility and Permeability Assessment of Biomaterials.Bioengineering (Basel, Switzerland) · 2024Article
- Preclinical Evaluation of the Oncolytic Vaccinia Virus TG6002 by Translational Research on Canine Breast Cancer.Molecular therapy oncolytics · 2020Article
- Intestinal Explant Cultures from Gilthead Seabream (International journal of molecular sciences · 2020Article
- Review
- Ex vivo treatment of patient biopsies as a novel method to assess colorectal tumour response to the MEK1/2 inhibitor, Selumetinib.Scientific reports · 2017Article
- The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies.Journal of visualized experiments : JoVE · 2017Article
- Human Organ Culture: Updating the Approach to Bridge the Gap fromFrontiers in medicine · 2017Review
- Tumor-selective proteotoxicity of verteporfin inhibits colon cancer progression independently of YAP1.Science signaling · 2015Article
- Transforming growth factor-β1 regulated phosphorylated AKT and interferon gamma expressions are associated with epithelial cell survival in rhesus macaque colon explants.Clinical immunology (Orlando, Fla.) · 2015Article
- Ex vivo culture of primary human colonic tissue for studying transcriptional responses to 1α,25(OH)2 and 25(OH) vitamin D.Physiological genomics · 2014Article
- Human colonic crypts in culture: segregation of immunochemical markers in normal versus adenoma-derived.Laboratory investigation; a journal of technical methods and pathology · 2014Article
- Article
- Interleukin-10 prevents epithelial cell apoptosis by regulating IFNγ and TNFα expression in rhesus macaque colon explants.Cytokine · 2013Article
- Altered molecular pattern of mucosal healing in Crohn's disease fibrotic stenosis.World journal of gastrointestinal pathophysiology · 2013Article
- Lactobacillus paracasei and Lactobacillus plantarum strains downregulate proinflammatory genes in an ex vivo system of cultured human colonic mucosa.Genes & nutrition · 2013Article
- Human colon tissue in organ culture: calcium and multi-mineral-induced mucosal differentiation.In vitro cellular & developmental biology. Animal · 2011Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Normal and neoplastic human colon tissue obtained at surgery was used to establish conditions for organ culture. Optimal conditions included an atmosphere of 5% CO2 and 95% O2; tissue partially submerged with mucosa at the gas interface; and serum-free medium with 1.5 mM Ca2+ and a number of growth supplements. Histological, histochemical, and immunohistochemical features that distinguish normal and neoplastic tissue were preserved over a 2-d period. With normal tissue, this included the presence of elongated crypts with small, densely packed cells at the crypt base and mucin-containing goblet cells in the upper portion. Ki67 staining, for proliferating cells, was confined to the lower third of the crypt, while expression of extracellular calcium-sensing receptor was seen in the upper third and surface epithelium. E-cadherin and β-catenin were expressed throughout the epithelium and confined to the cell surface. In tumor tissue, the same disorganized, abnormal glandular structures seen at time zero were present after 2 d. The majority of cells in these structures were mucin-poor, but occasional goblet cells were seen and mucin staining was present. Ki67 staining was seen throughout the abnormal epithelium and calcium-sensing receptor expression was weak and variable. E-cadherin was seen at the cell surface (similar to normal tissue), but in some places, there was diffuse cytoplasmic staining. Finally, intense cytoplasmic and nuclear β-catenin staining was observed in cultured neoplastic tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.