ArticleNature methods2009
Cell stimulation with optically manipulated microsources.
Article in Nature methods, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 94 citations in OpenAlex.
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- Large-scale control over collective cell migration using light-activated epidermal growth factor receptors.Cell systems · 2025Article
- Large-scale control over collective cell migration using light-controlled epidermal growth factor receptors.bioRxiv : the preprint server for biology · 2024Article
- Zero-order free holographic optical tweezers.Optics express · 2023Article
- The spatial resolution limit of phagocytosis.Biophysical journal · 2023Article
- Short-term bioelectric stimulation of collective cell migration in tissues reprograms long-term supracellular dynamics.PNAS nexus · 2022Article
- SCHEEPDOG: Programming Electric Cues to Dynamically Herd Large-Scale Cell Migration.Cell systems · 2020Article
- Large-scale patterning of living colloids for dynamic studies of neutrophil-microbe interactions.Lab on a chip · 2018Article
- Combining FRET and optical tweezers to study RhoGTPases spatio-temporal dynamics upon local stimulation.Journal of biological methods · 2017Article
- Porous microwells for geometry-selective, large-scale microparticle arrays.Nature materials · 2017Article
- Gelatin device for the delivery of growth factors involved in endochondral ossification.PloS one · 2017Article
- Light-driven micro-tool equipped with a syringe function.Light, science & applications · 2016Article
- Remote Control of T Cell Activation Using Magnetic Janus Particles.Angewandte Chemie (International ed. in English) · 2016Article
- Optical micromanipulation of nanoparticles and cells inside living zebrafish.Nature communications · 2016Article
- Optical Pushing: A Tool for Parallelized Biomolecule Manipulation.Biophysical journal · 2016Article
- Chemokine-Releasing Microparticles Improve Bacterial Clearance and Survival of Anthrax Spore-Challenged Mice.PloS one · 2016Article
- Optically-controlled platforms for transfection and single- and sub-cellular surgery.Biophysical reviews · 2015Review
- A dynamic model of chemoattractant-induced cell migration.Biophysical journal · 2015Article
- Chemokine-Releasing Nanoparticles for Manipulation of Lymph Node Microenvironment.Nanomaterials (Basel, Switzerland) · 2015Article
- De novo chemoattractants form supramolecular hydrogels for immunomodulating neutrophils in vivo.Bioconjugate chemistry · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
Molecular gradients are important for various biological processes including the polarization of tissues and cells during embryogenesis and chemotaxis. Investigations of these phenomena require control over the chemical microenvironment of cells. We present a technique to set up molecular concentration patterns that are chemically, spatially and temporally flexible. Our strategy uses optically manipulated microsources, which steadily release molecules. Our technique enables the control of molecular concentrations over length scales down to about 1 microm and timescales from fractions of a second to an hour. We demonstrate this technique by manipulating the motility of single human neutrophils. We induced directed cell polarization and migration with microsources loaded with the chemoattractant formyl-methionine-leucine-phenylalanine. Furthermore, we triggered highly localized retraction of lamellipodia and redirection of polarization and migration with microsources releasing cytochalasin D, an inhibitor of actin polymerization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.