ArticleNature chemical biology2009
Moonlighting proteins Hal3 and Vhs3 form a heteromeric PPCDC with Ykl088w in yeast CoA biosynthesis.
Article in Nature chemical biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 57 citations in OpenAlex.
- Potassium ion homeostasis modulates mitochondrial function.The Journal of cell biology · 2026Article
- Sis2 regulates yeast replicative lifespan in a dose-dependent manner.Nature communications · 2023Article
- Moonlighting Proteins: Diverse Functions Found in Fungi.Journal of fungi (Basel, Switzerland) · 2023Review
- Nascent peptide-induced translation discontinuation in eukaryotes impacts biased amino acid usage in proteomes.Nature communications · 2022Article
- Functional mapping of the N-terminal region of the yeast moonlighting protein Sis2/Hal3 reveals crucial residues for Ppz1 regulation.The FEBS journal · 2022Article
- Fungal Hal3 (and Its Close Relative Cab3) as Moonlighting Proteins.Journal of fungi (Basel, Switzerland) · 2022Review
- When Phosphatases Go Mad: The Molecular Basis for Toxicity of Yeast Ppz1.International journal of molecular sciences · 2022Review
- Comparative Analysis of Type 1 and Type Z Protein Phosphatases Reveals D615 as a Key Residue for Ppz1 Regulation.International journal of molecular sciences · 2022Article
- The Toxic Effects of Ppz1 Overexpression Involve Nha1-Mediated Deregulation of KJournal of fungi (Basel, Switzerland) · 2021Article
- Increased biosynthesis of acetyl-CoA in the yeast Saccharomyces cerevisiae by overexpression of a deregulated pantothenate kinase gene and engineering of the coenzyme A biosynthetic pathway.Applied microbiology and biotechnology · 2021Article
- Regulation of the CoA Biosynthetic Complex Assembly in Mammalian Cells.International journal of molecular sciences · 2021Article
- Inhibiting Mycobacterium tuberculosis CoaBC by targeting an allosteric site.Nature communications · 2021Article
- MdHAL3, a 4'-phosphopantothenoylcysteine decarboxylase, is involved in the salt tolerance of autotetraploid apple.Plant cell reports · 2020Article
- The N-Terminal Region of Yeast Protein Phosphatase Ppz1 Is a Determinant for Its Toxicity.International journal of molecular sciences · 2020Article
- Yeast Ppz1 protein phosphatase toxicity involves the alteration of multiple cellular targets.Scientific reports · 2020Article
- Protein Phosphatase Ppz1 Is Not Regulated by a Hal3-Like Protein in Plant PathogenInternational journal of molecular sciences · 2019Article
- Ser/Thr protein phosphatases in fungi: structure, regulation and function.Microbial cell (Graz, Austria) · 2019Review
- Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function.Scientific reports · 2018Article
- The inhibitory mechanism of Hal3 on the yeast Ppz1 phosphatase: A mutagenesis analysis.Scientific reports · 2017Article
- The Expanding Landscape of Moonlighting Proteins in Yeasts.Microbiology and molecular biology reviews : MMBR · 2016Review
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Unlike most other organisms, the essential five-step coenzyme A biosynthetic pathway has not been fully resolved in yeast. Specifically, the genes encoding the phosphopantothenoylcysteine decarboxylase (PPCDC) activity still remain unidentified. Sequence homology analyses suggest three candidates-Ykl088w, Hal3 and Vhs3-as putative PPCDC enzymes in Saccharomyces cerevisiae. Notably, Hal3 and Vhs3 have been characterized as negative regulatory subunits of the Ppz1 protein phosphatase. Here we show that YKL088w does not encode a third Ppz1 regulatory subunit, and that the essential roles of Ykl088w and the Hal3 and Vhs3 pair are complementary, cannot be interchanged and can be attributed to PPCDC-related functions. We demonstrate that while known eukaryotic PPCDCs are homotrimers, the active yeast enzyme is a heterotrimer that consists of Ykl088w and Hal3/Vhs3 monomers that separately provides two essential catalytic residues. Our results unveil Hal3 and Vhs3 as moonlighting proteins involved in both CoA biosynthesis and protein phosphatase regulation.
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