ArticleEukaryotic cell2010
abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.
Article in Eukaryotic cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 24 citations in OpenAlex.
- Functional characterization of Hul6 reveals a role in iron-dependent metabolism in Schizosaccharomyces pombe.Archives of microbiology · 2026Article
- Fission yeast cells deficient in siderophore biosynthesis require Str2 for ferrichrome-dependent growth.Frontiers in microbiology · 2025Article
- Structures and coordination chemistry of transporters involved in manganese and iron homeostasis.Biochemical Society transactions · 2023Review
- Regulation of iron homeostasis by peroxide-sensitive CatR, a Fur-family regulator in Streptomyces coelicolor.Journal of microbiology (Seoul, Korea) · 2021Article
- Non-mitochondrial aconitase regulates the expression of iron-uptake genes by controlling the RNA turnover process in fission yeast.Journal of microbiology (Seoul, Korea) · 2021Article
- Fungal-Metal Interactions: A Review of Toxicity and Homeostasis.Journal of fungi (Basel, Switzerland) · 2021Review
- Spore Germination Requires Ferrichrome Biosynthesis and the Siderophore Transporter Str1 inGenetics · 2019Article
- The major facilitator transporter Str3 is required for low-affinity heme acquisition inThe Journal of biological chemistry · 2018Article
- Metals in fungal virulence.FEMS microbiology reviews · 2018Review
- Heme Assimilation inThe Journal of biological chemistry · 2017Article
- Shu1 is a cell-surface protein involved in iron acquisition from heme in Schizosaccharomyces pombe.The Journal of biological chemistry · 2015Article
- Characterization of Schizosaccharomyces pombe copper transporter proteins in meiotic and sporulating cells.The Journal of biological chemistry · 2014Article
- Identification of genes expressed by Cryptococcus gattii during iron deprivation.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2014Article
- Metabolic remodeling in iron-deficient fungi.Biochimica et biophysica acta · 2012Review
- The ins and outs of algal metal transport.Biochimica et biophysica acta · 2012Review
- Grx4 monothiol glutaredoxin is required for iron limitation-dependent inhibition of Fep1.Eukaryotic cell · 2011Article
- Iron-dependent remodeling of fungal metabolic pathways associated with ferrichrome biosynthesis.Applied and environmental microbiology · 2010Article
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Studies have shown the fundamental contribution of the yeast vacuole as a site for storage and detoxification of metals. Whereas the transmembrane proteins responsible for iron transport into and out of the vacuole have been identified in Saccharomyces cerevisiae, less information is available concerning the mobilization of vacuolar iron stores in Schizosaccharomyces pombe. In this study, we report the identification of a gene designated abc3(+) that encodes a protein which exhibits sequence homology with the ABCC subfamily of ATP-binding cassette transporters. The transcription of abc3(+) is induced by low concentrations of iron but repressed by high levels of iron. The iron-mediated repression of abc3(+) required a functional fep1(+) gene. Chromatin immunoprecipitation assays showed that Fep1 associates with the abc3(+) promoter in vivo, in an iron-dependent manner. Microscopic analyses revealed that a functional Abc3-green fluorescent protein localizes to the membrane vacuole when iron levels were low. Abc3 was required for growth in low-iron medium in the absence of the transport system mediated by Fio1 and Fip1. abc3Delta cells exhibited increased levels of expression of the frp1(+)-encoded ferric reductase, suggesting a loss of Fep1 repression and, consequently, the activation of Fep1-regulated genes. When abc3(+) was expressed using the nmt1(+) promoter system, its induction led to a reduced transcriptional activity of the frp1(+) gene. Because S. pombe does not possess vacuolar membrane-localized orthologs to S. cerevisiae Fth1, Fet5, and Smf3, our findings suggested that Abc3 may be responsible for mobilizing stored iron from the vacuole to the cytosol in response to iron deficiency.
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