ArticleApplied and environmental microbiology2010
Quantification of cyprinid herpesvirus 3 in environmental water by using an external standard virus.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 49 citations in OpenAlex.
- Red Sea Bream Iridovirus (RSIV) Kinetics in Rock Bream (Animals : an open access journal from MDPI · 2022Article
- Quick detection ofFrontiers in cellular and infection microbiology · 2022Article
- Investigation of Cyprinid Herpesvirus 3 (CyHV-3) Disease Periods and Factors Influencing CyHV-3 Transmission in A Low Stocking Density Infection Trial.Animals : an open access journal from MDPI · 2021Article
- Surveillance of SARS-CoV-2 in Sewage Treatment Plants between January 2020 and July 2021 in Taiwan.Pathogens (Basel, Switzerland) · 2021Article
- Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm.Microbiology spectrum · 2021Article
- Detection of SARS-CoV-2 in wastewater in Japan during a COVID-19 outbreak.The Science of the total environment · 2021Article
- Investigation of bacterial diversity and pathogen abundances in gibel carp (Carassius auratus gibelio) ponds during a cyprinid herpesvirus 2 outbreak.MicrobiologyOpen · 2019Article
- Cyprinid viral diseases and vaccine development.Fish & shellfish immunology · 2018Review
- Evaluation of Daphnid Grazing on Microscopic Zoosporic Fungi by Using Comparative Threshold Cycle Quantitative PCR.Applied and environmental microbiology · 2016Article
- Cell Culture Isolation of Piscine Nodavirus (Betanodavirus) in Fish-Rearing Seawater.Applied and environmental microbiology · 2016Article
- Cyprinid herpesvirus 3: an interesting virus for applied and fundamental research.Veterinary research · 2013Review
- CyHV-3: the third cyprinid herpesvirus.Diseases of aquatic organisms · 2013Review
- Transmission dynamics of an emerging infectious disease in wildlife through host reproductive cycles.The ISME journal · 2011Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cyprinid herpesvirus 3 (CyHV-3), a lethal DNA virus that spreads in natural lakes and rivers, infects common carp and koi. We established a quantification method for CyHV-3 that includes a viral concentration method and quantitative PCR combined with an external standard virus. Viral concentration methods were compared using the cation-coated filter and ultrafiltration methods. The recovery of virus-like particles was similar for the two methods (cation-coated filter method, 44%+/-19%, n=3; ultrafiltration method, 50%+/-3%, n=3); however, the former method was faster and more suitable for routine determinations. The recovery of seeded CyHV-3 based on the cation-coated filter method varied by more than 3 orders of magnitude among the water samples. The recovery yield of CyHV-3 was significantly correlated with that of the seeded lambda phage, and the average ratio of lambda to the CyHV-3 recovery yield was 1.4, indicating that lambda is useful as an external standard virus for determining the recovery yield of CyHV-3. Therefore, to quantify CyHV-3 in environmental water, a known amount of lambda was added as an external standard virus to each water sample. Using this method, CyHV-3 DNA was detected in 6 of the 10 (60%) types of environmental water tested; the highest concentration of CyHV-3 DNA was 2x10(5) copies liter(-1). The lowest recovery limit of CyHV-3 DNA was 60 copies liter(-1). This method is practical for monitoring CyHV-3 abundance in environmental water.
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