ArticleCell2009
Singularity in polarization: rewiring yeast cells to make two buds.
Article in Cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
103 citing papers in PubMed.
- Role of Gα-MAPK interaction in mating ofMolecular biology of the cell · 2025Article
- Negative feedback equalizes polarity sites in a multi-budding yeast.Current biology : CB · 2025Article
- Vacuoles provide the source membrane for TORC1-containing signaling endosomes.The Journal of cell biology · 2025Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Redundancy and the role of protein copy numbers in the cell polarization machinery of budding yeast.Nature communications · 2023Article
- Particle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating.PLoS computational biology · 2023Article
- A gene duplication of a septin reveals a developmentally regulated filament length control mechanism.The Journal of cell biology · 2023Article
- Oligomerization of peripheral membrane proteins provides tunable control of cell surface polarity.Biophysical journal · 2022Article
- Mechanism of commitment to a mating partner inMolecular biology of the cell · 2022Article
- Unified multiscale theory of cellular mechanical adaptations to substrate stiffness.Biophysical journal · 2022Article
- Cdc42 GTPase-activating proteins (GAPs) regulate generational inheritance of cell polarity and cell shape in fission yeast.Molecular biology of the cell · 2021Article
- Chemotactic movement of a polarity site enables yeast cells to find their mates.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Exploratory polarization facilitates mating partner selection inMolecular biology of the cell · 2021Article
- Article
- Mechanisms that ensure monogamous mating inMolecular biology of the cell · 2021Article
- Connecting cell polarity signals to the cytokinetic machinery in yeast and metazoan cells.Cell cycle (Georgetown, Tex.) · 2021Review
- Saccharomyces spores are born prepolarized to outgrow away from spore-spore connections and penetrate the ascus wall.Yeast (Chichester, England) · 2021Article
- Review
- Compete or Coexist? Why the Same Mechanisms of Symmetry Breaking Can Yield Distinct Outcomes.Cells · 2020Review
- Auxin-induced signaling protein nanoclustering contributes to cell polarity formation.Nature communications · 2020Article
43 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
For budding yeast to ensure formation of only one bud, cells must polarize toward one, and only one, site. Polarity establishment involves the Rho family GTPase Cdc42, which concentrates at polarization sites via a positive feedback loop. To assess whether singularity is linked to the specific Cdc42 feedback loop, we disabled the yeast cell's endogenous amplification mechanism and synthetically rewired the cells to employ a different positive feedback loop. Rewired cells violated singularity, occasionally making two buds. Even cells that made only one bud sometimes initiated two clusters of Cdc42, but then one cluster became dominant. Mathematical modeling indicated that, given sufficient time, competition between clusters would promote singularity. In rewired cells, competition occurred slowly and sometimes failed to develop a single "winning" cluster before budding. Slowing competition in normal cells also allowed occasional formation of two buds, suggesting that singularity is enforced by rapid competition between Cdc42 clusters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.