ArticleEuropean journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences2010
Transport of chitosan-DNA nanoparticles in human intestinal M-cell model versus normal intestinal enterocytes.
Article in European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Transport Mechanisms of 2D Nanoparticles across a Human Follicle-Associated Epithelium Model.ACS omega · 2025Article
- Nanoplastics Enhance Transmembrane Transport and Uptake of Carcinogens: Transcriptional Changes and the Effects of Weathering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Application of Nanovaccines in Autoimmune Diseases.International journal of nanomedicine · 2024Review
- Intestinal Peyer's Patches: Structure, Function, and In Vitro Modeling.Tissue engineering and regenerative medicine · 2023Review
- Phosphatidylserine-mediated oral tolerance.Cellular immunology · 2023Article
- Assessing the interactions between nanoparticles and biological barriers in vitro: a new challenge for microscopy techniques in nanomedicine.European journal of histochemistry : EJH · 2022Article
- In Vitro Models of Biological Barriers for Nanomedical Research.International journal of molecular sciences · 2022Review
- Targeting the Gut Mucosal Immune System Using Nanomaterials.Pharmaceutics · 2021Review
- Nanochitosan: Commemorating the Metamorphosis of an ExoSkeletal Waste to a Versatile Nutraceutical.Nanomaterials (Basel, Switzerland) · 2021Review
- Using 3D gastrointestinal tract in vitro models with microfold cells and mucus secreting ability to assess the hazard of copper oxide nanomaterials.Journal of nanobiotechnology · 2019Article
- PEGylated Chitosan for Nonviral Aerosol and Mucosal Delivery of the CRISPR/Cas9 System in Vitro.Molecular pharmaceutics · 2018Article
- Effects of food-borne nanomaterials on gastrointestinal tissues and microbiota.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2018Review
- Role of nanoparticle size, shape and surface chemistry in oral drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2016Article
- Progress and future of in vitro models to study translocation of nanoparticles.Archives of toxicology · 2015Review
- Therapeutic applications of hydrogels in oral drug delivery.Expert opinion on drug delivery · 2014Review
- Nanotechnology: an effective tool for enhancing bioavailability and bioactivity of phytomedicine.Asian Pacific journal of tropical biomedicine · 2014Review
- Drug delivery systems, CNS protection, and the blood brain barrier.BioMed research international · 2014Review
- Glial cell line-derived neurotrophic factor gene delivery via a polyethylene imine grafted chitosan carrier.International journal of nanomedicine · 2014Article
- Supramolecular self-assembled nanoparticles mediate oral delivery of therapeutic TNF-α siRNA against systemic inflammation.Angewandte Chemie (International ed. in English) · 2013Article
- Development and characterization of chitosan-PEG-TAT nanoparticles for the intracellular delivery of siRNA.International journal of nanomedicine · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Oral vaccination is one of the most promising applications of polymeric nanoparticles. Using two different in vitro cellular models to partially reproduce the characteristics of intestinal enterocytes and M-cells, this study demonstrates that nanoparticle transport through the M-cell co-culture model is 5-fold that of the intestinal epithelial monolayer, with at least 80% of the chitosan-DNA nanoparticles uptaken in the first 30 min. Among the properties of nanoparticles studied, ligand decoration has the most dramatic effect on the transcytosis rate: transferrin modification enhances transport through both models by 3- to 5-fold. The stability of the nanoparticles also affects transport kinetics. Factors which de-stabilize the nanoparticles, such as low charge (N/P) ratio and addition of serum, result in aggregation and in turn decreases transport efficiency. Of these stability factors, luminal pH is of great interest as an increase in pH from 5.5 to 6.4 and 7.4 leads to a 3- and 10-fold drop in nanoparticle transport, respectively. Since soluble chitosan can act as an enhancer to increase paracellular transport by up to 60%, this decrease is partially attributed to the soluble chitosan precipitating near neutral pH. The implication that chitosan-DNA nanoparticles are more stable in the upper regions of the small intestine suggests that higher uptake rates may occur in the duodenum compared to the ileum and the colon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.