ArticleJournal of cellular and molecular medicine2009
Functionalized self-assembling peptide hydrogel enhance maintenance of hepatocyte activity in vitro.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Synthetic extracellular matrices with function-encoding peptides.Nature reviews bioengineering · 2023Review
- Increased Stiffness Downregulates Focal Adhesion Kinase Expression in Pancreatic Cancer Cells Cultured in 3D Self-Assembling Peptide Scaffolds.Biomedicines · 2022Article
- β-Sheet to Random Coil Transition in Self-Assembling Peptide Scaffolds Promotes Proteolytic Degradation.Biomolecules · 2022Article
- Culture and Differentiation of Human Hair Follicle Dermal Papilla Cells in a Soft 3D Self-Assembling Peptide Scaffold.Biomolecules · 2020Article
- HBV-related hepatocarcinogenesis: the role of signalling pathways and innovative ex vivo research models.BMC cancer · 2019Review
- Development of a 3D Co-Culture System as a Cancer Model Using a Self-Assembling Peptide Scaffold.Gels (Basel, Switzerland) · 2018Article
- A Pathway to Personalizing Therapy for Metastases Using Liver-on-a-Chip Platforms.Stem cell reviews and reports · 2017Review
- Dedifferentiated Human Articular Chondrocytes Redifferentiate to a Cartilage-Like Tissue Phenotype in a Poly(ε-Caprolactone)/Self-Assembling Peptide Composite Scaffold.Materials (Basel, Switzerland) · 2016Article
- Chondroitin Sulfate- and Decorin-Based Self-Assembling Scaffolds for Cartilage Tissue Engineering.PloS one · 2016Article
- Supramolecular Hydrogelators and Hydrogels: From Soft Matter to Molecular Biomaterials.Chemical reviews · 2015Review
- 3D Cultivation Techniques for Primary Human Hepatocytes.Microarrays (Basel, Switzerland) · 2015Review
- Long-term culture of primary hepatocytes: new matrices and microfluidic devices.Hepatology international · 2014Article
- Toward a 3D cellular model for studying in vitro the outcome of photodynamic treatments: accounting for the effects of tissue complexity.Tissue engineering. Part A · 2013Article
- Self-assembling peptide scaffolds as innovative platforms for drug and cell delivery systems in cardiac regeneration.Drug delivery and translational research · 2013Article
- Development of a three-dimensional bone-like construct in a soft self-assembling peptide matrix.Tissue engineering. Part A · 2013Article
- HelixSoft matter · 2012Article
- Regulation of endothelial cell activation and angiogenesis by injectable peptide nanofibers.Acta biomaterialia · 2012Article
- Engineering strategies to recapitulate epithelial morphogenesis within synthetic three-dimensional extracellular matrix with tunable mechanical properties.Physical biology · 2011Article
- Nanometric self-assembling peptide layers maintain adult hepatocyte phenotype in sandwich cultures.Journal of nanobiotechnology · 2010Article
- Synergistic effects of tethered growth factors and adhesion ligands on DNA synthesis and function of primary hepatocytes cultured on soft synthetic hydrogels.Biomaterials · 2010Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
There is a major challenge in maintaining functional hepatocytes in vivo as these cells rapidly lose their metabolic properties in culture. In this work we have developed a bioengineered platform that replaces the use of the collagen I--in the traditional culture sandwich technique--by a defined extracellular matrix analogue, the self-assembling peptide hydrogel RAD16-I functionalized with biologically active motifs. Thus, after examining side by side the two culture systems we have found that in both cases hepatocytes acquired similar parenchymal morphology, presence of functional bile canaliculi structures, CYP3A2 induction by dexamethasone, urea production, secretion of proteins such as apolipoprotein (class A1, E, J), alpha(1)-microglobulin, alpha(1)-macroglobulin, retinol binding protein, fibronectin, alpha(1)-inhibitor III and biotin-dependent carboxylases. Interestingly, by assessing in more detail some other hepatic markers, one of the functionalized matrix analogues--carrying the 67 kD laminin receptor ligand--enhanced the gene expression of albumin, HNF4-alpha, MDR2 and tyrosine aminotransferase. We conclude that the use of a synthetic culture system with designed matrix functionalization has the advantage in controlling specific cellular responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.