ArticleTissue engineering. Part A2010
Efficient procurement of epithelial stem cells from human tissue specimens using a Rho-associated protein kinase inhibitor Y-27632.
Article in Tissue engineering. Part A, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 75 citations in OpenAlex.
- Limitations of acyclovir and identification of potent HSV antivirals using 3D bioprinted human skin equivalents.Nature communications · 2025Article
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- De novo reconstruction of a functional in vivo-like equine endometrium using collagen-based tissue engineering.Scientific reports · 2024Article
- Y-27632 acts beyond ROCK inhibition to maintain epidermal stem-like cells in culture.Journal of cell science · 2023Article
- Article
- A novel cell-based transplantation method using a Rho kinase inhibitor and a specific catheter device for the treatment of salivary gland damage after head and neck radiotherapy.Biochemistry and biophysics reports · 2022Article
- A medium composition containing normal resting glucose that supports differentiation of primary human airway cells.Scientific reports · 2022Article
- Morphometric Analysis of Rat Prostate Development: Roles of MEK/ERK and Rho Signaling Pathways in Prostatic Morphogenesis.Biomolecules · 2021Article
- Laminin-511-derived recombinant fragment and Rho kinase inhibitor Y-27632 facilitate serial cultivation of keratinocytes differentiated from human embryonic stem cells.Regenerative therapy · 2021Article
- Article
- Conditional Cell Reprogramming in Modeling Digestive System Diseases.Frontiers in cell and developmental biology · 2021Review
- Establishment of an Efficient Primary Culture System for Human Hair Follicle Stem Cells Using the Rho-Associated Protein Kinase Inhibitor Y-27632.Frontiers in cell and developmental biology · 2021Article
- Conditional reprogramming: next generation cell culture.Acta pharmaceutica Sinica. B · 2020Review
- Progress in studies of epidermal stem cells and their application in skin tissue engineering.Stem cell research & therapy · 2020Review
- Review
- Long-term expansion of directly reprogrammed keratinocyte-like cells and in vitro reconstitution of human skin.Journal of biomedical science · 2020Article
- Establishment of ornithine transcarbamylase deficiency-derived primary human hepatocyte with hepatic functions.Experimental cell research · 2019Article
- Long-term culture and characterization of patient-derived primary hepatocytes using conditional reprogramming.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- RhoA controls retinoid signaling by ROCK dependent regulation of retinol metabolism.Small GTPases · 2018Article
- A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue.Journal of visualized experiments : JoVE · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The efficient culture of stem cells from epithelial tissues such as skin and corneas is important for both experimental studies and clinical applications of tissue engineering. We now demonstrate that treatment of human-skin-derived keratinocytes with a Rho-associated protein kinase inhibitor Y-27632 for the initial 6 days of primary culture can increase the number of keratinocytes that possess stem cell properties to form colonies during in vitro culture of freshly isolated cells and subsequent passage (50-fold). Further, we show that Y-27632 treatment can increase the total number of prostate epithelial cells derived from human prostate specimens. Therefore, the use of Y-27632 during primary cultures offers a simple and effective way to prepare a large number of epithelial stem cells from various human epithelial tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.