ArticleJournal of proteome research2010
Quantitative organelle proteomics of MCF-7 breast cancer cells reveals multiple subcellular locations for proteins in cellular functional processes.
Article in Journal of proteome research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Indocyanine Green as a Theragnostic Agent in MCF-7 Breast Cancer Cells.Molecules (Basel, Switzerland) · 2026Article
- Advances and Applications of Spatial Proteomics: From Organellar Maps to Clinical Translation.Chembiochem : a European journal of chemical biology · 2026Review
- Semi-supervised Bayesian integration of multiple spatial proteomics datasets.PLoS computational biology · 2025Article
- Differences in Protein Capture by SP3 and SP4 Demonstrate Mechanistic Insights of Proteomics Cleanup Techniques.Journal of proteome research · 2024Article
- Common methods in mitochondrial research (Review).International journal of molecular medicine · 2022Article
- Article
- Moonlighting Proteins and Cardiopathy in the Spatial Response of MCF-7 Breast Cancer Cells to Tamoxifen.Proteomics. Clinical applications · 2019Article
- Combining LOPIT with differential ultracentrifugation for high-resolution spatial proteomics.Nature communications · 2019Article
- Article
- LIN28A Modulates Splicing and Gene Expression Programs in Breast Cancer Cells.Molecular and cellular biology · 2015Article
- Comparative Analysis of Label-Free and 8-Plex iTRAQ Approach for Quantitative Tissue Proteomic Analysis.PloS one · 2015Article
- Involvement of Tetraspanin C189 in Cell-to-Cell Spreading of the Dengue Virus in C6/36 Cells.PLoS neglected tropical diseases · 2015Article
- Nuclear cytoplasmic trafficking of proteins is a major response of human fibroblasts to oxidative stress.Journal of proteome research · 2014Article
- Systematic nucleo-cytoplasmic trafficking of proteins following exposure of MCF7 breast cancer cells to estradiol.Journal of proteome research · 2014Article
- Subcellular proteomics reveals a role for nucleo-cytoplasmic trafficking at the DNA replication origin activation checkpoint.Journal of proteome research · 2013Article
- Spatial distribution of cellular function: the partitioning of proteins between mitochondria and the nucleus in MCF7 breast cancer cells.Journal of proteome research · 2012Article
- Proteomic analysis of lipid raft-enriched membranes isolated from internal organelles.Biochemical and biophysical research communications · 2011Article
- Quantitative subcellular proteome and secretome profiling of influenza A virus-infected human primary macrophages.PLoS pathogens · 2011Article
- Proteomic approaches to understanding the role of the cytoskeleton in host-defense mechanisms.Expert review of proteomics · 2011Review
- How Many proteins are Missed in Quantitative proteomics Based on Ms/Ms sequencing Methods?Proteomics insights · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
We have combined sucrose density gradient subcellular fractionation with quantitative, tandem-mass-spectrometry-based shotgun proteomics to investigate spatial distributions of proteins in MCF-7 breast cancer cells. Emphasis was placed on four major organellar compartments: cytosol, plasma membrane, endoplasmic reticulum, and mitochondrion. Two-thousand one-hundred eighty-four proteins were securely identified. Four-hundred eighty-one proteins (22.0% of total proteins identified) were found in unique sucrose gradient fractions, suggesting they may have unique subcellular locations. 454 proteins (20.8%) were found to be ubiquitously distributed. The remaining 1249 proteins (57.2%) were consistent with intermediate distribution over multiple, but not all, subcellular locations. Ninety-four proteins implicated in breast cancer and 478 other proteins which share the same five major cellular biological processes with a majority of the breast cancer proteins were observed in 334 and 1223 subcellular locations, respectively. The data obtained is used to evaluate the possibility of defining more exact sets of subcellular organelles, the completeness of current descriptions of spatial distribution of cellular proteins, the importance of multiple subcellular locations for proteins in functional processes, the subcellular distribution of proteins related to breast cancer, and the possibility of using these methods for dynamic spatio/temporal studies of function/regulation in MCF-7 breast cancer cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.