ArticleBiochemistry2009
Peptidomics of prolyl endopeptidase in the central nervous system.
Article in Biochemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- Exploring bi-carbazole-linked triazoles as inhibitors of prolyl endo peptidase via integrated in vitro and in silico study.Scientific reports · 2024Article
- DPP8/9 are not Required to Cleave Most Proline-Containing Peptides.Israel journal of chemistry · 2023Article
- Screening Trimethoprim Primary Metabolites for Covalent Binding to Albumin.Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents · 2020Article
- Alzheimer-associated cerebrospinal fluid fragments of neurogranin are generated by Calpain-1 and prolyl endopeptidase.Molecular neurodegeneration · 2018Article
- Mechanism of Action of Prolyl Oligopeptidase (PREP) in Degenerative Brain Diseases: Has Peptidase Activity Only a Modulatory Role on the Interactions of PREP with Proteins?Frontiers in aging neuroscience · 2017Article
- Inhibition of Prolyl Oligopeptidase Restores Spontaneous Motor Behavior in the α-Synuclein Virus Vector-Based Parkinson's Disease Mouse Model by Decreasing α-Synuclein Oligomeric Species in Mouse Brain.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Exploring metabolic pathways and regulation through functional chemoproteomic and metabolomic platforms.Chemistry & biology · 2014Review
- A novel secreted metalloprotease (CD2830) from Clostridium difficile cleaves specific proline sequences in LPXTG cell surface proteins.Molecular & cellular proteomics : MCP · 2014Article
- Proteasome inhibitors alter levels of intracellular peptides in HEK293T and SH-SY5Y cells.PloS one · 2014Article
- Analysis of the proteolysis of bioactive peptides using a peptidomics approach.Nature protocols · 2013Article
- Prolyl endopeptidase-deficient mice have reduced synaptic spine density in the CA1 region of the hippocampus, impaired LTP, and spatial learning and memory.Cerebral cortex (New York, N.Y. : 1991) · 2013Article
- Recent advances in quantitative neuroproteomics.Methods (San Diego, Calif.) · 2013Review
- Peptidomics methods for the identification of peptidase-substrate interactions.Current opinion in chemical biology · 2013Review
- Proteolysis controls endogenous substance P levels.PloS one · 2013Article
- Targeted modification of wheat grain protein to reduce the content of celiac causing epitopes.Functional & integrative genomics · 2012Review
- The pharmacological landscape and therapeutic potential of serine hydrolases.Nature reviews. Drug discovery · 2012Review
- The metabolic serine hydrolases and their functions in mammalian physiology and disease.Chemical reviews · 2011Review
- Investigating endogenous peptides and peptidases using peptidomics.Biochemistry · 2011Review
- A substrate-free activity-based protein profiling screen for the discovery of selective PREPL inhibitors.Journal of the American Chemical Society · 2011Article
- A peptidomics strategy to elucidate the proteolytic pathways that inactivate peptide hormones.Biochemistry · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Prolyl endopeptidase (Prep) is a member of the prolyl peptidase family and is of interest because of its unique biochemistry and connections to cognitive function. Using an unbiased mass spectrometry (MS)-based peptidomics platform, we identified Prep-regulated peptides in the central nervous system (CNS) of mice by measuring changes in the peptidome as a function of Prep activity. This approach was validated by the identification of known Prep substrates, such as the neuropeptide substance P and thymosin-beta4, the precursor to the bioactive peptide Ac-SDKP. In addition to these known substrates, we also discovered that Prep regulates many additional peptides, including additional bioactive peptides and proline rich peptides (PRPs). Biochemical experiments confirmed that some of these Prep-regulated peptides are indeed substrates of the enzyme. Moreover, these experiments also supported the known preference of Prep for shorter peptides while revealing a previously unknown cleavage site specificity of Prep when processing certain multi-proline-containing peptides, including PRPs. The discovery of Prep-regulated peptides implicates Prep in new biological pathways and provides insights into the biochemistry of this enzyme.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.