ArticleThe Journal of membrane biology2009
Cell migration in BeWo cells and the role of epithelial sodium channels.
Article in The Journal of membrane biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.Journal of cell science · 2013Trial
- Stable overexpression of the epithelial sodium channel alpha subunit reduces migration and proliferation in breast cancer cells.Breast cancer research and treatment · 2025Article
- Placental ion channels: potential target of chemical exposure.Biology of reproduction · 2023Review
- Epithelial sodium channels in macrophage migration and polarization: role of proinflammatory cytokines TNFα and IFNγ.American journal of physiology. Regulatory, integrative and comparative physiology · 2022Article
- The Effect of Dynasore Upon the Negative Interaction Between ENaC and CFTR Channels in Xenopus laevis Oocytes.The Journal of membrane biology · 2022Article
- Targeting Ion Channels for Cancer Treatment: Current Progress and Future Challenges.Reviews of physiology, biochemistry and pharmacology · 2022Review
- The epithelial sodium channel has a role in breast cancer cell proliferation.Breast cancer research and treatment · 2021Article
- Hyponatremia and Cancer Progression: Possible Association with Sodium-Transporting Proteins.Bioelectricity · 2020Article
- Sodium homeostasis in the tumour microenvironment.Biochimica et biophysica acta. Reviews on cancer · 2019Review
- Cell volume regulation in cancer cell migration driven by osmotic water flow.Cancer science · 2019Review
- The inflammatory cytokine TNF contributes with RAC3-induced malignant transformation.EXCLI journal · 2018Article
- The Epithelial Sodium Channel and the Processes of Wound Healing.BioMed research international · 2016Review
- Potential Roles of Amiloride-Sensitive Sodium Channels in Cancer Development.BioMed research international · 2016Review
- ENaC/DEG in Tumor Development and Progression.Journal of Cancer · 2016Review
- Physical and functional interactions between a glioma cation channel and integrin-β1 require α-actinin.American journal of physiology. Cell physiology · 2015Article
- Hydration status regulates sodium flux and inflammatory pathways through epithelial sodium channel (ENaC) in the skin.The Journal of investigative dermatology · 2015Article
- Heme oxygenase-1 promotes migration and β-epithelial Na+ channel expression in cytotrophoblasts and ischemic placentas.American journal of physiology. Regulatory, integrative and comparative physiology · 2014Article
- Article
- Hypotonic regulation of mouse epithelial sodium channel in Xenopus laevis oocytes.The Journal of membrane biology · 2013Article
- The migratory capacity of human trophoblastic BeWo cells: effects of aldosterone and the epithelial sodium channel.The Journal of membrane biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell migration/proliferation processes associated with wound healing were measured in BeWo cells at 6 h, when mitosis is still scarce. Cells were cultured in medium with 1% fetal bovine serum to minimize proliferation. BeWo cell migration covered 20.6 +/- 7.0%, 38.0 +/- 5.4%, 16.6 +/- 4.8% and 13.7 +/- 3.6% of the wound when cultivated under control, aldosterone (100 nM, 12 h), aldosterone plus amiloride (10 muM) and amiloride treatments, respectively. When BeWo cells were treated with aldosterone, there was an increase in wound healing (P < 0.05), which was prevented by adding the ENaC blocker amiloride (P < 0.05, n = 16). Immunocytochemistry studies showed that the three ENaC subunits showed greater expression at the leading edge of the wound 3 h after injury, supporting the notion that these proteins participate in a postinjury signal. Antisense oligonucleotides directed against the alpha-ENaC subunit decreased the migratory response of the cells compared to the sense treated cells or the cells without oligonucleotides (P < 0.001, n = 16): 30.2 +/- 3.7%, 17.6 +/- 1.3%, 27.5 +/- 1.5% and 20.2 +/- 1.5% reinvasion of the wound with aldosterone, aldosterone plus antisense, aldosterone plus sense treatments and control conditions, respectively. Aldosterone and amiloride influence wound healing in BeWo cells, probably by their effects upon ENaCs, transmitting a signal to the cell cytoplasm for the release of several agents that promote cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.