ArticleProceedings of the National Academy of Sciences of the United States of America2009
MAP kinases MPK9 and MPK12 are preferentially expressed in guard cells and positively regulate ROS-mediated ABA signaling.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 176 papers.
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176 citing papers in PubMed, 413 citations in OpenAlex.
- Guard cell signal transduction mechanisms controlling stomatal movements by light, abscisic acid, CO2, VPD and heat.Plant physiology · 2026Review
- Cell-type specific early perception of nine phytohormones revealed by single-nucleus transcriptomics in Arabidopsis.Nature communications · 2026Article
- The MPK6-WRKY49 module links MAPK signaling to ABA biosynthesis and drought tolerance in Arabidopsis.The Plant journal : for cell and molecular biology · 2026Article
- Dissecting the ROS signalling component of salinity tolerance: tissue-specific K+/Na+ homeostasis in quinoa and spinach roots.Journal of experimental botany · 2026Article
- MITOGEN-ACTIVATED PROTEIN KINASE9 fine-tunes hypoxia signaling by phosphorylating ERF-VII transcription factors in Arabidopsis.The New phytologist · 2026Article
- Elucidation of the genetic basis underlying rooting ability in vegetatively propagated chrysanthemum.Horticulture research · 2026Article
- Unveiling the early defense response dynamics in grapevines against Plasmopara viticola by single-cell transcriptomics.Genome biology · 2026Article
- Genome-Wide Analysis of Cellulose Synthase Superfamily and Roles ofPlants (Basel, Switzerland) · 2025Article
- GmMEKK2 Disrupts the MKK1/2-MPK4 Cascade to Amplify Immune Signalling and Confer Enhanced Resistance to Soybean Mosaic Virus.Molecular plant pathology · 2025Article
- Article
- Stomatal signaling under drought: integrating hormonal pathways for crop resilience.Plant cell reports · 2025Review
- Transcriptome analysis reveals key defense genes associated with resistance to banana blood disease.BMC genomics · 2025Article
- The Apple Mitogen-Activated Protein KinaseInternational journal of molecular sciences · 2025Article
- Glycolysis and signal transduction participate in Lycium barbarum in response to NaCl stress through protein phosphorylation.BMC plant biology · 2025Article
- The Arabidopsis F-box protein FBS associated with the helix-loop-helix transcription factor FAMA involved in stomatal immunity.Plant molecular biology · 2025Article
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- Genome-wide screening of mitogen-activated protein kinase (MAPK) gene family and expression profile under heavy metal stress in Solanum lycopersicum.Biotechnology letters · 2025Article
- Antioxidants by nature: an ancient feature at the heart of flavonoids' multifunctionality.The New phytologist · 2025Article
- Molecular traits of MAPK kinases and the regulatory mechanism of GhMAPKK5 alleviating drought/salt stress in cotton.Plant physiology · 2024Article
- Multi-omics analyses reveal the mechanisms underlying the responses of Casuarina equisetifolia ssp. incana to seawater atomization and encroachment stress.BMC plant biology · 2024Article
116 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 7 institutions in 7 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species (ROS) mediate abscisic acid (ABA) signaling in guard cells. To dissect guard cell ABA-ROS signaling genetically, a cell type-specific functional genomics approach was used to identify 2 MAPK genes, MPK9 and MPK12, which are preferentially and highly expressed in guard cells. To provide genetic evidence for their function, Arabidopsis single and double TILLING mutants that carry deleterious point mutations in these genes were isolated. RNAi-based gene-silencing plant lines, in which both genes are silenced simultaneously, were generated also. Mutants carrying a mutation in only 1 of these genes did not show any altered phenotype, indicating functional redundancy in these genes. ABA-induced stomatal closure was strongly impaired in 2 independent RNAi lines in which both MPK9 and MPK12 transcripts were significantly silenced. Consistent with this result, mpk9-1/12-1 double mutants showed an enhanced transpirational water loss and ABA- and H(2)O(2)-insensitive stomatal response. Furthermore, ABA and calcium failed to activate anion channels in guard cells of mpk9-1/12-1, indicating that these 2 MPKs act upstream of anion channels in guard cell ABA signaling. An MPK12-YFP fusion construct rescued the ABA-insensitive stomatal response phenotype of mpk9-1/12-1, demonstrating that the phenotype was caused by the mutations. The MPK12 protein is localized in the cytosol and the nucleus, and ABA and H(2)O(2) treatments enhance the protein kinase activity of MPK12. Together, these results provide genetic evidence that MPK9 and MPK12 function downstream of ROS to regulate guard cell ABA signaling positively.
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