ArticleJournal of magnetic resonance (San Diego, Calif. : 1997)2010
A bioreactor for in-cell protein NMR.
Article in Journal of magnetic resonance (San Diego, Calif. : 1997), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- In-cell NMR spectroscopy: advancements, applications, challenges, and future directions in structural biology.Magma (New York, N.Y.) · 2026Review
- Quantitative Competition Binding of Fluorinated Ligands by Real-TimeMagnetic resonance in chemistry : MRC · 2026Article
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- From molecular descriptions to cellular functions of intrinsically disordered protein regions.Biophysics reviews · 2024Review
- Ligand-Based Competition Binding by Real-TimeJournal of medicinal chemistry · 2024Article
- Real-Time Monitoring of RAS Activity Using In Vitro and In-Cell NMR Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins.Journal of biomolecular NMR · 2023Article
- Protein structure determination in human cells by in-cell NMR and a reporter system to optimize protein delivery or transexpression.Communications biology · 2022Article
- Radio Signals from Live Cells: The Coming of Age of In-Cell Solution NMR.Chemical reviews · 2022Review
- Towards Profiling of the G-Quadruplex Targeting Drugs in the Living Human Cells Using NMR Spectroscopy.International journal of molecular sciences · 2021Article
- Protein in-cell NMR spectroscopy at 1.2 GHz.Journal of biomolecular NMR · 2021Article
- Applications of Solution NMR in Drug Discovery.Molecules (Basel, Switzerland) · 2021Review
- A Nuclear Magnetic Resonance (NMR) Platform for Real-Time Metabolic Monitoring of Bioprocesses.Molecules (Basel, Switzerland) · 2020Article
- Real-Time Quantitative In-Cell NMR: Ligand Binding and Protein Oxidation Monitored in Human Cells Using Multivariate Curve Resolution.Analytical chemistry · 2020Article
- Active metabolism unmasks functional protein-protein interactions in real time in-cell NMR.Communications biology · 2020Article
- NMR Characterization of Surface Receptor Protein Interactions in Live Cells Using Methylcellulose Hydrogels.Angewandte Chemie (International ed. in English) · 2020Article
- In-Cell NMR of Intrinsically Disordered Proteins in Mammalian Cells.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Observation of nucleic acids inside living human cells by in-cell NMR spectroscopy.Biophysics and physicobiology · 2020Article
- Protein Structure Determination in Living Cells.International journal of molecular sciences · 2019Review
- In-Cell NMR Spectroscopy of Intrinsically Disordered Proteins.Proteomics · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
The inside of the cell is a complex environment that is difficult to simulate when studying proteins and other molecules in vitro. We have developed a device and system that provides a controlled environment for nuclear magnetic resonance (NMR) experiments involving living cells. Our device comprises two main parts, an NMR detection region and a circulation system. The flow of medium from the bottom of the device pushes alginate encapsulated cells into the circulation chamber. In the chamber, the exchange of oxygen and nutrients occurs between the media and the encapsulated cells. When the media flow is stopped, the encapsulated cells fall back into the NMR detection region, and spectra can be acquired. We have utilized the bioreactor to study the expression of the natively disordered protein alpha-synuclein, inside Escherichia coli cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.