ArticleAnalytical chemistry2009
Fluorescent marker for direct detection of specific dsDNA sequences.
Article in Analytical chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Advancing Multicolor Super-Resolution Volume Imaging: Illuminating Complex Cellular Dynamics.JACS Au · 2025Review
- Conditions for a microfluidic creep experiment for microparticles using a cross-slot extensional flow device.Biomicrofluidics · 2025Article
- Applications of triplex DNA nanostructures in sensor development.Analytical and bioanalytical chemistry · 2022Review
- Restriction Endonuclease-Based Assays for DNA Detection and Isothermal Exponential Signal Amplification.Sensors (Basel, Switzerland) · 2020Article
- Sensitive Fluorescent Sensor for Recognition of HIV-1 dsDNA by Using Glucose Oxidase and Triplex DNA.Journal of analytical methods in chemistry · 2018Article
- Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR.Scientific reports · 2015Article
- Insights into copper coordination in the EcoRI-DNA complex by ESR spectroscopy.Molecular physics · 2014Article
- Toward single-molecule optical mapping of the epigenome.ACS nano · 2014Article
- Sensitive Cu2+-Cu2+ distance measurements in a protein-DNA complex by double-quantum coherence ESR.The journal of physical chemistry. B · 2013Article
- Simulating the dynamics and orientations of spin-labeled side chains in a protein-DNA complex.The journal of physical chemistry. B · 2012Article
- Polymer-monovalent salt-induced DNA compaction studied via single-molecule microfluidic trapping.Lab on a chip · 2012Article
- Labeling DNA for single-molecule experiments: methods of labeling internal specific sequences on double-stranded DNA.Nanoscale · 2011Review
- Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping.Lab on a chip · 2011Article
- Single-molecule sequence detection via microfluidic planar extensional flow at a stagnation point.Lab on a chip · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We have created a fluorescent marker using a mutant EcoRI restriction endonuclease (K249C) that enables prolonged, direct visualization of specific sequences on genomic lengths of double-stranded (ds) DNA. The marker consists of a biotinylated enzyme, attached through the biotin-avidin interaction to a fluorescent nanosphere. Control over biotin position with respect to the enzyme's binding pocket is achieved by biotinylating the mutant EcoRI at the mutation site. Biotinylated enzyme is incubated with dsDNA and NeutrAvidin-coated, fluorescent nanospheres under conditions that allow enzyme binding but prevent cleavage. Marker-laden DNA is then fluorescently stained and stretched on polylysine-coated glass slides so that the positions of the bound markers along individual DNA molecules can be measured. We demonstrate the marker's ability to bind specifically to its target sequence using both bulk gel-shift assays and single-molecule methods.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.