ArticleJournal of the American Chemical Society2009
FRET enabled real time detection of RNA-small molecule binding.
Article in Journal of the American Chemical Society, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
- Isomorphic Fluorescent Nucleosides.Accounts of chemical research · 2024Article
- Targeting MicroRNAs with Small Molecules.Non-coding RNA · 2024Review
- Interbase-FRET binding assay for pre-microRNAs.Scientific reports · 2021Article
- Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase-Modified Fluorogenic RNA Aptamers.Angewandte Chemie (International ed. in English) · 2020Article
- Interbase FRET in RNA: from A to Z.Nucleic acids research · 2019Article
- Fluorescence-based investigations of RNA-small molecule interactions.Methods (San Diego, Calif.) · 2019Article
- RNA Structural Differentiation: Opportunities with Pattern Recognition.Biochemistry · 2019Review
- Differentiation and classification of RNA motifs using small molecule-based pattern recognition.Methods in enzymology · 2019Article
- 2-Allyl- and Propargylamino-dATPs for Site-Specific Enzymatic Introduction of a Single Modification in the Minor Groove of DNA.Chemistry (Weinheim an der Bergstrasse, Germany) · 2018Article
- HgII binds to C-T mismatches with high affinity.Nucleic acids research · 2018Article
- Emissive Synthetic Cofactors: Enzymatic Interconversions ofAngewandte Chemie (International ed. in English) · 2018Article
- Enzymatic incorporation and utilization of an emissive 6-azauridine.Organic & biomolecular chemistry · 2017Article
- Studies of RNA Sequence and Structure Using Nanopores.Progress in molecular biology and translational science · 2016Review
- TEMPO-derived spin labels linked to the nucleobases adenine and cytosine for probing local structural perturbations in DNA by EPR spectroscopy.Beilstein journal of organic chemistry · 2015Article
- Singly modified amikacin and tobramycin derivatives show increased rRNA A-site binding and higher potency against resistant bacteria.ChemMedChem · 2014Article
- Polymyxins and analogues bind to ribosomal RNA and interfere with eukaryotic translation in vitro.Chembiochem : a European journal of chemical biology · 2013Article
- Oligodeoxynucleotides containing multiple thiophene-modified isomorphic fluorescent nucleosides.The Journal of organic chemistry · 2013Article
- A fluorescence-based screen for ribosome binding antibiotics.Analytical biochemistry · 2013Article
- Direct observation of aminoglycoside-RNA binding by localized surface plasmon resonance spectroscopy.Analytical chemistry · 2013Article
- Modified 6-aza uridines: highly emissive pH-sensitive fluorescent nucleosides.Chemphyschem : a European journal of chemical physics and physical chemistry · 2012Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A robust analysis and discovery platform for antibiotics targeting the bacterial rRNA A-site has been developed by incorporating a new emissive U surrogate into the RNA and labeling the aminoglycosides with an appropriate fluorescence acceptor. Specifically, a 5-methoxyquinazoline-2,4(1H,3H)-dione-based emissive uracil analogue was identified to be an ideal donor for 7-diethylaminocoumarin-3-carboxylic acid. This donor/acceptor pair displays a critical Forster radius (R(0)) of 27 A, a value suitable for an A-site-aminoglycoside assembly. Titrating the coumarin labeled aminoglycosides into the emissive A-site construct, labeled at position U1406, shows a decrease in donor emission (at 395 nm) and concurrent increase of the acceptor emission (at 473 nm). Titration curves, obtained by fitting the donor's emission quenching or the augmentation of the acceptor's sensitized emission, faithfully generate EC(50) values. Titration of unlabeled ligands into the preformed FRET complex showed a continuous increase of the donor emission, with a concurrent decrease of the acceptor emission, yielding valuable data regarding competitive displacement of aminoglycosides by A-site binders. Detection of antibiotic binding is therefore not dependent on changes in the environment of a single fluorophore, but rather on the responsive interaction between two chromophores acting as a FRET pair, facilitating the determination of direct binding and competitive displacement events with FRET accuracy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.