ArticleMethods in molecular biology (Clifton, N.J.)2010
Directed differentiation of human embryonic stem cells to epidermal progenitors.
Article in Methods in molecular biology (Clifton, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- YAP-TEAD regulates the super-enhancer network to control early surface ectoderm commitment.Nucleic acids research · 2026Article
- The combined effects of Map3k1 mutation and dioxin on differentiation of keratinocytes derived from mouse embryonic stem cells.Scientific reports · 2022Article
- Bioactive Molecules for Skin Repair and Regeneration: Progress and Perspectives.Stem cells international · 2019Review
- Top-Down Inhibition of BMP Signaling Enables Robust Induction of hPSCs Into Neural Crest in Fully Defined, Xeno-free Conditions.Stem cell reports · 2017Article
- Directed differentiation of human embryonic stem cells into keratinocyte progenitors in vitro: an attempt with promise of clinical use.In vitro cellular & developmental biology. Animal · 2016Article
- Generation of Epithelial Cell Populations from Human Pluripotent Stem Cells Using a Small-Molecule Inhibitor of Src Family Kinases.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Transcriptional profiling of ectoderm specification to keratinocyte fate in human embryonic stem cells.PloS one · 2015Article
- Efficient generation of functional epithelial and epidermal cells from human pluripotent stem cells under defined conditions.Tissue engineering. Part C, Methods · 2013Article
- Engineering the human pluripotent stem cell microenvironment to direct cell fate.Biotechnology advances · 2013Review
- Improving efficiency of human pluripotent stem cell differentiation platforms using an integrated experimental and computational approach.Biotechnology and bioengineering · 2013Article
- Lectin-functionalized microchannels for characterizing pluripotent cells and early differentiation.Biomicrofluidics · 2012Article
- Donor cell type can influence the epigenome and differentiation potential of human induced pluripotent stem cells.Nature biotechnology · 2011Article
- Induction of putative stratified epithelial progenitor cells in vitro from mouse-induced pluripotent stem cells.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2011Article
- Generation of stratified squamous epithelial progenitor cells from mouse induced pluripotent stem cells.PloS one · 2011Article
- Investigation of K14/K5 as a stem cell marker in the limbal region of the bovine cornea.PloS one · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Human embryonic stem (hES) cells can differentiate into virtually all somatic cell types. In order to incorporate these derivatives into scientific or clinical applications, efficient methods of directing hES cell differentiation to pure subpopulations are required. Here we describe a robust strategy for generating cytokeratin 14+ (K14+)/p63+ keratinocyte progenitors from hES cells through stage-specific application of retinoic acid (RA) and bone morphogenetic protein-4 (BMP4). Induction of undifferentiated hES cells with RA stimulates expression of epithelial genes such as K18 and p63. Subculture of RA-treated cells in defined keratinocyte medium enables isolation of relatively pure K14+ epithelial populations; these cells also retain the capacity to terminally differentiate. The use of defined media throughout differentiation allows for detailed characterization of keratinocyte lineage specification from hES cells through the use of gene expression and immunofluorescence analyses.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.