Evidence map›Paper›PMID 19906898›Full record

ArticleJournal of clinical microbiology2010

Commercial DNA probes for mycobacteria incorrectly identify a number of less frequently encountered species.

Enrico Tortoli, Monica Pecorari, Giuliana Fabio, Massimino Messinò, Anna Fabio

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Treatment of Nontuberculous Mycobacterial Pulmonary Disease: An Official ATS/ERS/ESCMID/IDSA Clinical Practice Guideline.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020
    Guideline
  2. Guideline
  3. Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Pulmonary infection byFrontiers in medicine · 2024
    Article
  9. Diagnostic and Therapeutic Challenges in DisseminatedOpen forum infectious diseases · 2023
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Misidentification ofJournal of clinical tuberculosis and other mycobacterial diseases · 2021
    Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Enrico TortoliRegional Reference Center for Mycobacteria, Piastra dei Servizi, A. O. U. Careggi, viale Morgagni 85, 50134 Florence, Italy. e.tortoli@libero.it
Monica Pecorari
Giuliana Fabio
Massimino Messinò
Anna Fabio

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with GenoType Mycobacterium (Hein), 317 strains, belonging to 136 species, 61 of which had never been assayed before. INNO LiPA misidentified 20 taxa, the majority of which cross-reacted with the probes specific for Mycobacterium fortuitum and the Mycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceum group. GenoType misidentified 28 taxa, most of which cross-reacted with M. intracellulare and M. fortuitum probes; furthermore, eight species were not recognized as members of the genus Mycobacterium. Among 54 strains investigated with AccuProbe (Gen-Probe), cross-reactions were detected for nine species, with the probes aiming at the M. avium complex being most involved in cross-reactions.

Indexed as

Diagnostic ErrorsBacteriological TechniquesCross ReactionsDNA, BacterialDNA ProbesHumansMolecular Diagnostic TechniquesMycobacteriumMycobacterium InfectionsReagent Kits, DiagnosticSensitivity and SpecificityDNA, BacterialDNA ProbesReagent Kits, Diagnostic

Identifiers

PMID19906898
PMCPMC2812265

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.