ArticlePlant physiology2010
Large-scale reverse genetics in Arabidopsis: case studies from the Chloroplast 2010 Project.
Article in Plant physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Plastid acyl carrier proteins play redundant roles in seed oil accumulation with functional differentiation on the fatty acid flux.Plant cell reports · 2026Article
- A data-integrative modeling approach accurately characterizes the effects of mutations on Arabidopsis lipid metabolism.Plant physiology · 2025Article
- Identification of a common secondary mutation in theMicrobiology spectrum · 2023Article
- Understanding protein import in diverse non-green plastids.Frontiers in genetics · 2023Review
- Lipidomic Analysis of Arabidopsis T-DNA Insertion Lines Leads to Identification and Characterization of C-Terminal Alterations in FATTY ACID DESATURASE 6.Plant & cell physiology · 2022Article
- A Plastid-Bound Ankyrin Repeat Protein Controls Gametophyte and Early Embryo Development inFrontiers in plant science · 2022Article
- An Argon-Ion-Induced Pale Green Mutant ofPlants (Basel, Switzerland) · 2021Article
- A Novel Chloroplast Protein RNA Processing 8 Is Required for the Expression of Chloroplast Genes and Chloroplast Development inFrontiers in plant science · 2021Article
- Mitochondrial Fatty Acid Synthase Utilizes Multiple Acyl Carrier Protein Isoforms.Plant physiology · 2020Article
- Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants.Scientific reports · 2020Article
- Challenges and opportunities for strain verification by whole-genome sequencing.Scientific reports · 2020Article
- A simple, non-toxic method for separating seeds based on density, and its application in isolatingApplications in plant sciences · 2020Article
- Article
- A predicted plastid rhomboid protease affects phosphatidic acid metabolism in Arabidopsis thaliana.The Plant journal : for cell and molecular biology · 2019Article
- Characterization of Proteins Involved in Chloroplast Targeting Disturbed by Rice Stripe Virus by Novel Protoplast⁻Chloroplast Proteomics.International journal of molecular sciences · 2019Article
- Two Abscisic Acid-Responsive Plastid Lipase Genes Involved in Jasmonic Acid Biosynthesis inThe Plant cell · 2018Article
- Chlorophyll Fluorescence Video Imaging: A Versatile Tool for Identifying Factors Related to Photosynthesis.Frontiers in plant science · 2018Review
- A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Root Cell-Specific Regulators of Phosphate-Dependent Growth.Plant physiology · 2017Article
- A Plastid Phosphatidylglycerol Lipase Contributes to the Export of Acyl Groups from Plastids for Seed Oil Biosynthesis.The Plant cell · 2017Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Traditionally, phenotype-driven forward genetic plant mutant studies have been among the most successful approaches to revealing the roles of genes and their products and elucidating biochemical, developmental, and signaling pathways. A limitation is that it is time consuming, and sometimes technically challenging, to discover the gene responsible for a phenotype by map-based cloning or discovery of the insertion element. Reverse genetics is also an excellent way to associate genes with phenotypes, although an absence of detectable phenotypes often results when screening a small number of mutants with a limited range of phenotypic assays. The Arabidopsis Chloroplast 2010 Project (www.plastid.msu.edu) seeks synergy between forward and reverse genetics by screening thousands of sequence-indexed Arabidopsis (Arabidopsis thaliana) T-DNA insertion mutants for a diverse set of phenotypes. Results from this project are discussed that highlight the strengths and limitations of the approach. We describe the discovery of altered fatty acid desaturation phenotypes associated with mutants of At1g10310, previously described as a pterin aldehyde reductase in folate metabolism. Data are presented to show that growth, fatty acid, and chlorophyll fluorescence defects previously associated with antisense inhibition of synthesis of the family of acyl carrier proteins can be attributed to a single gene insertion in Acyl Carrier Protein4 (At4g25050). A variety of cautionary examples associated with the use of sequence-indexed T-DNA mutants are described, including the need to genotype all lines chosen for analysis (even when they number in the thousands) and the presence of tagged and untagged secondary mutations that can lead to the observed phenotypes.
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