Evidence map›Paper›PMID 19906719›Full record

ArticleNucleic acids research2010

PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification.

Athanasia Spandidos, Xiaowei Wang, Huajun Wang, Brian Seed

Abstract read
In one paragraph

Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 608 papers.

0numbers the graph read from it
0cells of the map it votes in
608citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

608 citing papers in PubMed.

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  14. Translational pediatrics · 2026
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548 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Athanasia SpandidosDepartment of Genetics, Harvard Medical School, Center for Computational and Integrative Biology, Massachusetts General Hospital, Boston, MA 02114-2790, USA.
Xiaowei Wang
Huajun Wang
Brian Seed

Funding

GENOMIC ANALYSIS OF STRESS AND INFLAMMATIONU01HL066678 · NHLBI · MASSACHUSETTS GENERAL HOSPITAL · PI FREEMAN, MASON W · 2000 to 2004
$14.9M
COMBINED COMPUTATIONAL AND EXPERIMENTAL ANALYSES OF GENE REGULATION BY MICRORNAS.R01GM089784 · NIGMS · WASHINGTON UNIVERSITY · PI WANG, XIAOWEI · 2010 to 2019
$2.8M
NHLBI NIH HHS U01 HL66678NIGMS NIH HHS R01 GM089784
6 · The paper itself

Abstract

PrimerBank (http://pga.mgh.harvard.edu/primerbank/) is a public resource for the retrieval of human and mouse primer pairs for gene expression analysis by PCR and Quantitative PCR (QPCR). A total of 306,800 primers covering most known human and mouse genes can be accessed from the PrimerBank database, together with information on these primers such as T(m), location on the transcript and amplicon size. For each gene, at least one primer pair has been designed and in many cases alternative primer pairs exist. Primers have been designed to work under the same PCR conditions, thus facilitating high-throughput QPCR. There are several ways to search for primers for the gene(s) of interest, such as by: GenBank accession number, NCBI protein accession number, NCBI gene ID, PrimerBank ID, NCBI gene symbol or gene description (keyword). In all, 26,855 primer pairs covering most known mouse genes have been experimentally validated by QPCR, agarose gel analysis, sequencing and BLAST, and all validation data can be freely accessed from the PrimerBank web site.

Indexed as

Databases, GeneticDatabases, Nucleic AcidGene Expression ProfilingAnimalsBase SequenceComputational BiologyDatabases, ProteinDNA PrimersHumansInformation Storage and RetrievalInternetMiceMolecular Sequence DataPolymerase Chain ReactionSoftwareDNA Primers

Identifiers

PMID19906719
PMCPMC2808898

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.