ArticleBMC infectious diseases2009
PCR melting profile (PCR MP)--a new tool for differentiation of Candida albicans strains.
Article in BMC infectious diseases, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Candida albicans Carriage in Children with Severe Early Childhood Caries (S-ECC) and Maternal Relatedness.PloS one · 2016Article
- PCR melting profile as a tool for outbreak studies of Salmonella enterica in chickens.BMC veterinary research · 2015Article
- Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland.Memorias do Instituto Oswaldo Cruz · 2014Article
- High-resolution melting-curve analysis of ligation-mediated real-time PCR for rapid evaluation of an epidemiological outbreak of extended-spectrum-beta-lactamase-producing Escherichia coli.Journal of clinical microbiology · 2011Article
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5 authors.
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Abstract
backgroundWe have previously reported the use of PCR Melting Profile (PCR MP) technique based on using low denaturation temperatures during ligation mediated PCR (LM PCR) for bacterial strain differentiation. The aim of the current study was to evaluate this method for intra-species differentiation of Candida albicans strains.
methodsIn total 123 Candida albicans strains (including 7 reference, 11 clinical unrelated, and 105 isolates from patients of two hospitals in Poland) were examined using three genotyping methods: PCR MP, macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis (REA-PFGE) and RAPD techniques.
resultsThe genotyping results of the PCR MP were compared with results from REA-PFGE and RAPD techniques giving 27, 26 and 25 unique types, respectively. The results showed that the PCR MP technique has at least the same discriminatory power as REA-PFGE and RAPD.
conclusionData presented here show for the first time the evaluation of PCR MP technique for candidial strains differentiation and we propose that this can be used as a relatively simple and cheap technique for epidemiological studies in short period of time in hospital.
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