ArticleASN neuro2009
Dynamic imaging of cannabinoid receptor 1 vesicular trafficking in cultured astrocytes.
Article in ASN neuro, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Envisioning Cannabinoid CB1 Receptor Biased Signaling as a Therapeutic Target for Schizophrenia.The American journal of psychiatry · 2026Review
- Two Metabolic Fuels, Glucose and Lactate, Differentially Modulate Exocytotic Glutamate Release from Cultured Astrocytes.Neurochemical research · 2021Article
- Physiology of Astroglia.Physiological reviews · 2018Review
- Astrocyte Aquaporin Dynamics in Health and Disease.International journal of molecular sciences · 2016Review
- Connecting Malfunctioning Glial Cells and Brain Degenerative Disorders.Genomics, proteomics & bioinformatics · 2016Review
- Astrocytes as secretory cells of the central nervous system: idiosyncrasies of vesicular secretion.The EMBO journal · 2016Review
- Gene Electrotransfer: A Mechanistic Perspective.Current gene therapy · 2016Review
- Article
- GABAρ subunits confer a bicuculline-insensitive component to GFAP+ cells of cerebellum.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Subcellular localization of coagulation factor II receptor-like 1 in neurons governs angiogenesis.Nature medicine · 2014Article
- Intracellular tracking of single-plasmid DNA particles after delivery by electroporation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Astrocytic vesicle mobility in health and disease.International journal of molecular sciences · 2013Review
- Method for the determination of trajectory angles of directional secretory vesicles in cultured astrocytes.Inquiro · 2013Article
- Exocytosis in astrocytes: transmitter release and membrane signal regulation.Neurochemical research · 2012Review
- Revisiting CB1 receptor as drug target in human melanoma.Pathology oncology research : POR · 2012Article
- Astroglial excitability and gliotransmission: an appraisal of Ca2+ as a signalling route.ASN neuro · 2012Review
- Cannabinoid receptor expression in HIV encephalitis and HIV-associated neuropathologic comorbidities.Neuropathology and applied neurobiology · 2011Article
- Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes.American journal of physiology. Cell physiology · 2011Article
- Regulated exocytosis in astrocytic signal integration.Neurochemistry international · 2010Review
- Filming the glial dreams: real-time imaging of cannabinoid receptor trafficking in astrocytes.ASN neuro · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Astrocytes possess GPCRs (G-protein-coupled receptors) for neuroactive substances and can respond via these receptors to signals originating from neurons as well as astrocytes. Like many transmembrane proteins, GPCRs exist in a dynamic equilibrium between receptors expressed at the plasma membrane and those present within intracellular trafficking compartments. The characteristics of GPCR trafficking within astrocytes have not been investigated. We therefore monitored the trafficking of recombinant fluorescent protein chimeras of the CB1R (cannabinoid receptor 1) that is thought to be expressed natively in astrocytes. CB1R chimeras displayed a marked punctate intracellular localization when expressed in cultured rat visual cortex astrocytes, an expression pattern reminiscent of native CB1R expression in these cells. Based upon trafficking characteristics, we found the existence of two populations of vesicular CB1R puncta: (i) relatively immobile puncta with movement characteristic of diffusion and (ii) mobile puncta with movement characteristic of active transport along cytoskeletal elements. The predominant direction of active transport is oriented radially to/from the nuclear region, which can be abolished by disruption of the microtubule cytoskeleton. CB1R puncta are localized within intracellular acidic organelles, mainly co-localizing with endocytic compartments. Constitutive trafficking of CB1R to and from the plasma membrane is an energetically costly endeavour whose function is at present unclear in astrocytes. However, given that intracellular CB1Rs can engage cell signalling pathways, it is likely that this process plays an important regulatory role.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.