ArticleStem cells (Dayton, Ohio)2010
Excision of reprogramming transgenes improves the differentiation potential of iPS cells generated with a single excisable vector.
Article in Stem cells (Dayton, Ohio), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 131 papers, 1 of them a synthesis that pooled it.
What it found
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Who cites it
131 citing papers in PubMed, 1 synthesis or guideline pooled it, 268 citations in OpenAlex.
- Induced pluripotent stem cells as a new getaway for bone tissue engineering: A systematic review.Cell proliferation · 2017Pooled it
- Generation of human alveolar epithelial type I cells from pluripotent stem cells.Cell stem cell · 2024Article
- Generation of iPSCs Using Sendai Virus Vectors.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Deconvoluting the Cells of the Human Heart with iPSC Technology: Cell Types, Protocols, and Uses.Current cardiology reports · 2022Review
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- An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool.Stem cells international · 2022Article
- A familiar study on self-limited childhood epilepsy patients using hIPSC-derived neurons shows a bias towards immaturity at the morphological, electrophysiological and gene expression levels.Stem cell research & therapy · 2021Article
- Chromosomal aberration arises during somatic reprogramming to pluripotent stem cells.Cell division · 2020Article
- Cell-Based Therapy Manufacturing in Stirred Suspension Bioreactor: Thoughts for cGMP Compliance.Frontiers in bioengineering and biotechnology · 2020Review
- Induced Pluripotent Stem Cell Derivation and Ex Vivo Gene Correction Using a Mucopolysaccharidosis Type 1 Disease Mouse Model.Stem cells international · 2019Article
- Fine-Tuning Mybl2 Is Required for Proper Mesenchymal-to-Epithelial Transition during Somatic Reprogramming.Cell reports · 2018Article
- Generation of Human Induced Pluripotent Stem Cells Using a Defined, Feeder-Free Reprogramming System.Current protocols in stem cell biology · 2018Article
- Autologous stem cell therapy for inherited and acquired retinal disease.Regenerative medicine · 2018Review
- Immortalized prairie vole-derived fibroblasts (VMF-K4DTs) can be transformed into pluripotent stem cells and provide a useful tool with which to determine optimal reprogramming conditions.The Journal of reproduction and development · 2017Article
- Induced pluripotent stem cell: A headway in reprogramming with promising approach in regenerative biology.Veterinary world · 2017Review
- Rhesus Macaque iPSC Generation and Maintenance.Current protocols in stem cell biology · 2017Article
- Pluripotent stem cells to hepatocytes, the journey so far.Biomedical reports · 2017Article
- Systematic evaluation of markers used for the identification of human induced pluripotent stem cells.Biology open · 2017Article
- CRISPR Genome Engineering for Human Pluripotent Stem Cell Research.Theranostics · 2017Review
71 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The residual presence of integrated transgenes following the derivation of induced pluripotent stem (iPS) cells is highly undesirable. Here we demonstrate efficient derivation of iPS cells free of exogenous reprogramming transgenes using an excisable polycistronic lentiviral vector. A novel version of this vector containing a reporter fluorochrome allows direct visualization of vector excision in living iPS cells in real time. We find that removal of the reprogramming vector markedly improves the developmental potential of iPS cells and significantly augments their capacity to undergo directed differentiation in vitro. We further propose that methods to efficiently excise reprogramming transgenes with minimal culture passaging, such as those demonstrated here, are critical since we find that iPS cells may acquire chromosomal abnormalities, such as trisomy of chromosome 8, similar to embryonic stem cells after expansion in culture. Our findings illustrate an efficient method for the generation of transgene-free iPS cells and emphasize the potential beneficial effects that may result from elimination of integrated reprogramming factors. In addition, our results underscore the consequences of long-term culture that will need to be taken into account for the clinical application of iPS cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.