ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2010
Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches.
Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Temporal myc dynamics permit mitotic bypass, promoting polyploid phenotypes.Cancer letters · 2025Review
- Genome-Wide Mapping of Autonomously Replicating Sequences in the Marine Diatom Phaeodactylum tricornutum.Marine biotechnology (New York, N.Y.) · 2024Article
- The impact of replication stress on replication dynamics and DNA damage in vertebrate cells.Nature reviews. Genetics · 2017Review
- Temporal association of ORCA/LRWD1 to late-firing origins during G1 dictates heterochromatin replication and organization.Nucleic acids research · 2017Article
- Order from clutter: selective interactions at mammalian replication origins.Nature reviews. Genetics · 2017Review
- A replicator-specific binding protein essential for site-specific initiation of DNA replication in mammalian cells.Nature communications · 2016Article
- Replication landscape of the human genome.Nature communications · 2016Article
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- Recent advances in the genome-wide study of DNA replication origins in yeast.Frontiers in microbiology · 2015Article
- Best practices for mapping replication origins in eukaryotic chromosomes.Current protocols in cell biology · 2014Review
- Epigenetic landscape for initiation of DNA replication.Chromosoma · 2014Review
- Basal level of FANCD2 monoubiquitination is required for the maintenance of a sufficient number of licensed-replication origins to fire at a normal rate.Oncotarget · 2014Article
- Genome-wide mapping of human DNA-replication origins: levels of transcription at ORC1 sites regulate origin selection and replication timing.Genome research · 2013Article
- Open chromatin structures regulate the efficiencies of pre-RC formation and replication initiation in Epstein-Barr virus.The Journal of cell biology · 2012Article
- Human telomeres replicate using chromosome-specific, rather than universal, replication programs.The Journal of cell biology · 2012Article
- Different roles of the human Orc6 protein in the replication initiation process.Cellular and molecular life sciences : CMLS · 2011Article
- Pre-replication complex proteins assemble at regions of low nucleosome occupancy within the Chinese hamster dihydrofolate reductase initiation zone.Nucleic acids research · 2011Article
- Genome-wide mapping of Arabidopsis thaliana origins of DNA replication and their associated epigenetic marks.Nature structural & molecular biology · 2011Article
- Genomic approaches to the initiation of DNA replication and chromatin structure reveal a complex relationship.Briefings in functional genomics · 2011Review
- The dyad symmetry element of Epstein-Barr virus is a dominant but dispensable replication origin.PloS one · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA replication initiates from origins of replication following a strict sequential activation programme and a conserved temporal order of activation. The number of replication initiation sites varies between species, according to the complexity of the genomes, with an average spacing of 100,000 bp. In contrast to yeast genomes, the location and definition of origins in mammalian genomes has been elusive. Historically, mammalian replication initiation sites have been mapped in situ by systematically searching specific genomic loci for sites that preferentially initiated DNA replication, potential origins by start-site mapping and autonomously replicating sequence experiments, and potential ORC and pre-replicative complex (pre-RC) sites by chromatin immunoprecipitation (ChIP) using antibodies for pre-RC proteins. In the past decade, ChIP has become an important method for analyzing protein/DNA interactions. Classically, ChIP is combined with Southern blotting or PCR. Recently, whole genome-ChIP methods have been very successful in unicellular eukaryotes to understand molecular mechanisms coordinating replication initiation and its flexibility in response to environmental changes. However, in mammalian systems, ChIP with pre-RC antibodies has often been challenging and genome-wide studies are scarce. In this review, we will appraise the progress that has been made in understanding replication origin organization using immunoprecipitation of the ORC and Mcm2-7 complexes. A special focus will be on the advantages and disadvantages of genome-wide ChIP-technologies and their potential impact on understanding metazoan replicators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.