ArticleProceedings of the National Academy of Sciences of the United States of America2009
MRPS18-2 protein immortalizes primary rat embryonic fibroblasts and endows them with stem cell-like properties.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The role of DGAT1 and DGAT2 in regulating tumor cell growth and their potential clinical implications.Journal of translational medicine · 2024Review
- Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A novel role for DGATs in cancer.Advances in biological regulation · 2019Review
- The MRPS18-2 protein levels correlate with prostate tumor progression and it induces CXCR4-dependent migration of cancer cells.Scientific reports · 2018Article
- Rat embryonic fibroblasts immortalized by MRPS18-2 protein are target for NK-cells.Oncotarget · 2017Article
- Upregulation of Mrps18a in breast cancer identified by selecting phage antibody libraries on breast tissue sections.BMC cancer · 2017Article
- An Overview of Lipid Droplets in Cancer and Cancer Stem Cells.Stem cells international · 2017Review
- S18 family of mitochondrial ribosomal proteins: evolutionary history and Gly132 polymorphism in colon carcinoma.Oncotarget · 2016Article
- Mitochondrial ribosomal protein S18-2 is highly expressed in endometrial cancers along with free E2F1.Oncotarget · 2016Article
- Mitochondrial ribosomal protein S18-2 evokes chromosomal instability and transforms primary rat skin fibroblasts.Oncotarget · 2015Article
- Ribosomal proteins and human diseases: pathogenesis, molecular mechanisms, and therapeutic implications.Medicinal research reviews · 2015Review
- Overexpression of MRPS18-2 in Cancer Cell Lines Results in Appearance of Multinucleated Cells.Acta naturae · 2013Article
- Stem cell gene expression in MRPS18-2-immortalized rat embryonic fibroblasts.Cell death & disease · 2012Article
- Article
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7 authors.
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Abstract
We report that the overexpression of mitochondrial ribosomal protein MRPS18-2 (S18-2) can immortalize primary rat embryonic fibroblasts (REFs). The immortalized cells (18IM) lose contact inhibition, form foci, and are capable of anchorage-independent growth. Concurrently, mesodermal markers, such as vimentin, smooth muscle actin, and Fut4, disappear completely. 18IM cells express embryonic stem cell markers, such as SSEA-1, Sox2, and Oct3/4. In confluent cultures, a portion of cells also express ectoderm- and endoderm-specific pan-keratin, ectoderm-specific beta-III-tubulin, mesoderm-specific MHC class II, and become stainable for fat with Oil red O. None of these changes was detected in c-myc+Ha-ras (MR)-transformed cells. In immunodeficient mice, 18IM cells formed small transiently growing tumors that have down-regulated SSEA-1 and showed pan-keratin staining. We conclude that S18-2 can immortalize REFs and induces them to express stem cell traits.
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