ArticleThe journal of physical chemistry. B2010
Synchronously amplified fluorescence image recovery (SAFIRe).
Article in The journal of physical chemistry. B, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 46 citations in OpenAlex.
- Non-Covalent Reversibly Photoconvertible Fluorescent Tags for Wash-Free Protein Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- All-optical strategies to minimize photobleaching in reversibly switchable fluorescent proteins.Nature communications · 2025Article
- Fluorescence-informed photoacoustic discrimination of multiple chromophores by lifetime mapping optically gated responses.Photoacoustics · 2023Article
- Resonances at Fundamental and Harmonic Frequencies for Selective Imaging of Sine-Wave Illuminated Reversibly Photoactivatable Labels.Chemphyschem : a European journal of chemical physics and physical chemistry · 2022Article
- Extra kinetic dimensions for label discrimination.Nature communications · 2022Article
- Optically Modulated and Optically Activated Delayed Fluorescent Proteins through Dark State Engineering.The journal of physical chemistry. B · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging Under Adverse Optical Conditions.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Synchronously Amplified Photoacoustic Image Recovery (SAPhIRe).Photoacoustics · 2020Article
- Dynamic contrast with reversibly photoswitchable fluorescent labels for imaging living cells.Chemical science · 2020Review
- Facile autofluorescence suppression enabling tracking of single viruses in live cells.The Journal of biological chemistry · 2019Article
- Optically Activated Delayed Fluorescence.The journal of physical chemistry letters · 2017Article
- Improved Fluorescent Protein Contrast and Discrimination by Optically Controlling Dark State Lifetimes.The journal of physical chemistry letters · 2017Article
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
- Dark State-Modulated Fluorescence Correlation Spectroscopy for Quantitative Signal Recovery.The journal of physical chemistry letters · 2016Article
- Signal Improvement Strategies for Fluorescence Detection of Biomacromolecules.Journal of fluorescence · 2016Review
- Expanding discriminative dimensions for analysis and imaging.Chemical science · 2015Article
- Tailoring cyanine dark states for improved optically modulated fluorescence recovery.The journal of physical chemistry. B · 2015Article
- Optically modulated fluorescence bioimaging: visualizing obscured fluorophores in high background.Accounts of chemical research · 2014Review
- Fluorescence nanoscopy by polarization modulation and polarization angle narrowing.Nature methods · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Fluorescence intermittency severely limits brightness in both single molecule and bulk fluorescence. Herein, we demonstrate that optical depopulation of organic fluorophore triplet states opens a path to significantly increased sensitivity by simultaneously increasing brightness and greatly reducing background through synchronously detected fluorescence modulation. Image recovery is achieved through selective fluorescence enhancement via modulating a secondary laser excitation at much lower energy than the observed emission in order to depopulate the long-lived triplet states. A series of xanthene dyes that exhibit efficient triplet-state formation demonstrate that this method of selective signal extraction can be achieved at moderate primary and secondary excitation intensities through tailoring dye photophysics and imaging conditions. Up to 5-fold increases in solution-based fluorescence over primary laser excitation alone was achieved upon secondary laser excitation, and dynamic control of signal modulation was demonstrated over a wide time range simply by varying the modulation frequency of the laser used for depopulation of the triplet state. We identify the photophysical characteristics that enable existing or to-be-designed fluorophores to be used in synchronously amplified fluorescence image recovery (SAFIRe) microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.