ArticleParasitology research2010
A nested PCR assay exhibits enhanced sensitivity for detection of Theileria parva infections in bovine blood samples from carrier animals.
Article in Parasitology research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 66 citations in OpenAlex.
- Unveiling the tick-borne pathogens from domestic ruminant ticks in Malawi and the emergence of the brown ear tick in the southern region: implications for East Coast fever control.Parasite (Paris, France) · 2026Article
- Molecular epidemiology ofFrontiers in microbiology · 2026Article
- Molecular epidemiology and phylogenetic relationships of Theileria spp. in cattle from Marathwada region of Maharashtra state, India.Tropical animal health and production · 2025Article
- Theileria lestoquardi and malignant theileriosis in small ruminants: an updated review.Tropical animal health and production · 2025Review
- Molecular detection and phylogenetic analysis of tick-borne pathogens in cattle from southern Malawi.Veterinary research communications · 2024Article
- Tick populations and molecular detection of selected tick-borne pathogens in questing ticks from northern and central Tanzania.Experimental & applied acarology · 2023Article
- Conservation and variation in the region of the Theileria parva p104 antigen coding gene used for PCR surveillance of the parasite.Parasitology research · 2023Article
- East Coast Fever Carrier Status andPathogens (Basel, Switzerland) · 2023Article
- Assessment of the Impact of Early Diagnosis and Early Treatment in the Integrated Control of East Coast Fever (ECF) Involving Acquired Immunity Induced by Natural Infection in Ankole Cattle.Pathogens (Basel, Switzerland) · 2023Article
- A locus conferring tolerance to Theileria infection in African cattle.PLoS genetics · 2022Article
- A cross-sectional survey to establish Theileria parva prevalence and vector control at the wildlife-livestock interface, Northern Tanzania.Preventive veterinary medicine · 2021Article
- Prevalence of tick-transmitted pathogens in cattle reveals that Theileria parva, Babesia bigemina and Anaplasma marginale are endemic in Burundi.Parasites & vectors · 2021Article
- Inherited Tolerance in Cattle to the Apicomplexan ProtozoanFrontiers in cellular and infection microbiology · 2021Article
- Antigenic Diversity inFrontiers in genetics · 2021Article
- The Rhipicephalus appendiculatus tick vector of Theileria parva is absent from cape buffalo (Syncerus caffer) populations and associated ecosystems in northern Uganda.Parasitology research · 2020Article
- Genetic Diversity and Sequence Polymorphism of Two Genes Encoding Theileria parva Antigens Recognized by CD8Pathogens (Basel, Switzerland) · 2020Article
- Investigations into the carrier-state ofInternational journal for parasitology. Parasites and wildlife · 2020Article
- First detection of Theileria parva in cattle from Cameroon in the absence of the main tick vector Rhipicephalus appendiculatus.Transboundary and emerging diseases · 2020Article
- An appraisal of oriental theileriosis and the Theileria orientalis complex, with an emphasis on diagnosis and genetic characterisation.Parasitology research · 2020Review
- Genetic and antigenic variation of the bovine tick-borne pathogen Theileria parva in the Great Lakes region of Central Africa.Parasites & vectors · 2019Article
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Theileria parva causes East Coast fever, an economically important disease of cattle in sub-Saharan Africa. We describe a nested polymerase chain reaction (nPCR) assay for the detection of T. parva DNA in cattle blood spotted onto filter paper using primers derived from the T. parva-specific 104-kDa antigen (p104) gene. The sensitivity of this assay was compared to a previously described p104-based PCR and also the reverse line blot (RLB) technique, using serial dilutions of blood from a calf with known T. parva piroplasm parasitaemia. The relative sensitivities of the three assays were 0.4, 1.4 and 4 parasites/microl corresponding to blood parasitaemias of 9.2 x 10(-6)%, 2.8 x 10(-5)% and 8.3 x 10(-5)%, respectively. The three assays were applied to samples from two calves infected with the T. parva Muguga stock. Parasite DNA was consistently detectable by the two p104 PCR assays until 48 and 82 days post-infection, respectively, and thereafter sporadically. RLB detected parasite DNA in the two infected calves until days 43 and 45. Field samples from 151 Kenyan cattle exhibited 37.7% positivity for T. parva by regular p104 PCR and 42.3% positivity using p104 nPCR. Among 169 cattle blood samples from Southern Sudan, 36% were positive for T. parva using nPCR. The nPCR assay represents a highly sensitive tool for detection and monitoring of asymptomatic carrier state infections of T. parva in the blood of cattle.
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