ArticleCellular and molecular life sciences : CMLS2010
Visualization of subcellular NAD pools and intra-organellar protein localization by poly-ADP-ribose formation.
Article in Cellular and molecular life sciences : CMLS, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- Decreased mitochondrial NAD+ in WRN deficient cells links to dysfunctional proliferation.Aging · 2025Article
- Subcellular NADNature metabolism · 2024Article
- Accounting for NAD Concentrations in Genome-Scale Metabolic Models Captures Important Metabolic Alterations in NAD-Depleted Systems.Biomolecules · 2024Article
- Roles of NADCold Spring Harbor perspectives in medicine · 2024Review
- Review
- NADCell systems · 2021Article
- Welcome to the Family: Identification of the NADBiomolecules · 2021Review
- Review
- NADSignal transduction and targeted therapy · 2021Review
- SLC25A51 is a mammalian mitochondrial NADNature · 2020Article
- Location, Location, Location: Compartmentalization of NADTrends in biochemical sciences · 2020Review
- Minireview Exploring the Biological Cycle of Vitamin B3 and Its Influence on Oxidative Stress: Further Molecular and Clinical Aspects.Molecules (Basel, Switzerland) · 2020Review
- Non-invasive Optical Biomarkers Distinguish and Track the Metabolic Status of Single Hematopoietic Stem Cells.iScience · 2020Article
- The DNA-damage response and nuclear events as regulators of nonapoptotic forms of cell death.Oncogene · 2020Review
- Article
- NADCell metabolism · 2019Review
- Regulation of Glucose Metabolism by NADCells · 2019Review
- Subcellular compartmentalization of NADPharmacology & therapeutics · 2019Review
- Flow Cytometry Analysis of Free Intracellular NADCurrent protocols in cytometry · 2019Article
- The Effect of Exercise on the Skin Content of the Reduced Form of NAD and Its Response to Transient Ischemia and Reperfusion in Highly Trained Athletes.Frontiers in physiology · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly-ADP-ribose polymerases (PARPs) use NAD(+) as substrate to generate polymers of ADP-ribose. We targeted the catalytic domain of human PARP1 as molecular NAD(+) detector into cellular organelles. Immunochemical detection of polymers demonstrated distinct subcellular NAD(+) pools in mitochondria, peroxisomes and, surprisingly, in the endoplasmic reticulum and the Golgi complex. Polymers did not accumulate within the mitochondrial intermembrane space or the cytosol. We demonstrate the suitability of this compartment-specific NAD(+) and poly-ADP-ribose turnover to establish intra-organellar protein localization. For overexpressed proteins, genetically endowed with PARP activity, detection of polymers indicates segregation from the cytosol and consequently intra-organellar residence. In mitochondria, polymer build-up reveals matrix localization of the PARP fusion protein. Compared to presently used fusion tags for subcellular protein localization, these are substantial improvements in resolution. We thus established a novel molecular tool applicable for studies of subcellular NAD metabolism and protein localization.
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