ArticleOptometry and vision science : official publication of the American Academy of Optometry2010
Tear lipids interfacial rheology: effect of lysozyme and lens care solutions.
Article in Optometry and vision science : official publication of the American Academy of Optometry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 34 citations in OpenAlex.
- Viscoelasticity in 3D Cell Culture and Regenerative Medicine: Measurement Techniques and Biological Relevance.ACS materials Au · 2024Review
- Biophysical properties of tear film lipid layer I. Surface tension and surface rheology.Biophysical journal · 2022Article
- Recent Developments of Nanostructures for the Ocular Delivery of Natural Compounds.Frontiers in chemistry · 2022Review
- Recent advances in topical nano drug-delivery systems for the anterior ocular segment.Therapeutic delivery · 2018Review
- TFOS DEWS II Tear Film Report.The ocular surface · 2017Review
- Article
- Racial variations in interfacial behavior of lipids extracted from worn soft contact lenses.Optometry and vision science : official publication of the American Academy of Optometry · 2013Article
- Lipidomics of human Meibomian gland secretions: Chemistry, biophysics, and physiological role of Meibomian lipids.Progress in lipid research · 2011Review
- Lacritin proteoforms prevent tear film collapse and maintain epithelial homeostasis.The Journal of biological chemistryArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo evaluate the interfacial properties of ex vivo tear lipid multilayers with controlled and varying thickness. The influence of lysozyme and surfactant-containing multipurpose lens care solution (MPS) on interfacial rheology of lipids and mixed lipid-protein films were studied.
methodsLipids were extracted from lotrafilcon A lenses worn continuously for 1 month. Interfacial properties of the lipids without and with lysozyme in the aqueous phase were examined using tensiometry and step-strain relaxation. Lipid-lysozyme multilayers were exposed to either diluted opti-free express (OFX) or opti-free replenish (OFR) for 30 min, and then MPS was displaced from the bulk phase. Surface tension and rheological parameters before and after MPS exposure were measured and compared.
resultsThick lipid films exerted high surface pressure at the air-aqueous interface, 50 +/- 2 mN/m, with little inter- and intrasubject variability. The rheological storage modulus (E(infinity); 25.3 +/- 2 mN/m) and relaxation time (tau; 87 +/- 25 s) were similar among subjects. Neither lysozyme nor MPS changed the surface tension of the lipid multilayers. Lysozyme adsorbed irreversibly onto multilayers without changing E(infinity), but increased tau 2.5 times. Exposure of mixed multilayers to OFX reduced E(infinity) to less than a half of its original value (13 +/- 4.5 mN/m; p < 0.001), whereas after OFR exposure, small but statistically significant changes in E(infinity) were found (21 +/- 3.0 mN/m; p = 0.0044). MPS-treated mixed films relaxed much slower than an untreated one, tau increased 4.5 times after OFX and 7 times after OFR exposure.
conclusionsThick multilayers of the ex vivo tear lipids have exhibited surface tension 15 +/- 1 mN/m lower than reported in the literature for meibomian lipids. The lipid-lysozyme interaction altered the interfacial rheology of the ex vivo lipids. OFX and OFR changed rheological properties of the mixed films to different extents.
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