ArticleThe Journal of biological chemistry2010
Recognition of adenosine residues by the active site of poly(A)-specific ribonuclease.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- The RNA-Binding Protein PARN Remodeled 3' UTR Structure Defines Poly(A)-Loading Sites to Mediate Immunoglobulin Homeostasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- RNA-binding proteins and exoribonucleases modulating miRNA in cancer: the enemy within.Experimental & molecular medicine · 2024Review
- Regulation of human telomerase RNA biogenesis and localization.RNA biology · 2021Review
- Regulation of poly(a)-specific ribonuclease activity by reversible lysine acetylation.The Journal of biological chemistry · 2020Article
- Regulation of human telomerase in homeostasis and disease.Nature reviews. Molecular cell biology · 2020Review
- From incomplete penetrance with normal telomere length to severe disease and telomere shortening in a family with monoallelic and biallelic PARN pathogenic variants.Human mutation · 2019Article
- Article
- Disruption of Telomerase RNA Maturation Kinetics Precipitates Disease.Molecular cell · 2019Article
- The H/ACA complex disrupts triplex in hTR precursor to permit processing by RRP6 and PARN.Nature communications · 2018Article
- Analysis of mRNA deadenylation by multi-protein complexes.Methods (San Diego, Calif.) · 2017Article
- Poly(A)-specific ribonuclease is a nuclear ribosome biogenesis factor involved in human 18S rRNA maturation.Nucleic acids research · 2017Article
- Mammalian PNLDC1 is a novel poly(A) specific exonuclease with discrete expression during early development.Nucleic acids research · 2016Article
- Destabilization of microRNAs in human cells by 3' deadenylation mediated by PARN and CUGBP1.Nucleic acids research · 2015Article
- Structural insights into the stabilization of MALAT1 noncoding RNA by a bipartite triple helix.Nature structural & molecular biology · 2014Article
- An integrated in silico approach to design specific inhibitors targeting human poly(a)-specific ribonuclease.PloS one · 2012Article
- A deadenylase assay by size-exclusion chromatography.PloS one · 2012Article
- PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human mitochondria.Nucleic acids research · 2011Article
- A novel member of the RNase D exoribonuclease family functions in mitochondrial guide RNA metabolism in Trypanosoma brucei.The Journal of biological chemistry · 2011Article
- Molecular Interplay of PARN and Telomerase: Tail Modifiers and Disease Implications.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly(A)-specific ribonuclease (PARN) is a mammalian 3'-exoribonuclease that degrades poly(A) with high specificity. To reveal mechanisms by which poly(A) is recognized by the active site of PARN, we have performed a kinetic analysis using a large repertoire of trinucleotide substrates. Our analysis demonstrated that PARN harbors specificity for adenosine recognition in its active site and that the nucleotides surrounding the scissile bond are critical for adenosine recognition. We propose that two binding pockets, which interact with the nucleotides surrounding the scissile bond, play a pivotal role in providing specificity for the recognition of adenosine residues by the active site of PARN. In addition, we show that PARN, besides poly(A), also quite efficiently degrades poly(U), approximately 10-fold less efficiently than poly(A). The poly(U)-degrading property of PARN could be of biological significance as oligo(U) tails recently have been proposed to play a role in RNA stabilization and destabilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.