ArticleJournal of cancer research and clinical oncology2010
Features of senescence and cell death induced by doxorubicin in A549 cells: organization and level of selected cytoskeletal proteins.
Article in Journal of cancer research and clinical oncology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Senescent Alveolospheres: A Preliminary 3D Model for Exploring Epithelial Senescence and Pro-Fibrotic Signaling.International journal of molecular sciences · 2026Article
- Insulin-stimulated glucose uptake is impaired in senescent human adipocytes.Frontiers in endocrinology · 2026Article
- Acridine BasedMolecules (Basel, Switzerland) · 2022Article
- A new function for the serine protease HtrA2 in controlling radiation-induced senescence in cancer cells.Molecular oncology · 2022Article
- Ezh2 Inhibits Replicative Senescence of Atrial Fibroblasts Through Promotion of H3K27me3 in the Promoter Regions of CDKN2a and Timp4 Genes.Journal of inflammation research · 2022Article
- BH3 mimetics selectively eliminate chemotherapy-induced senescent cells and improve response in TP53 wild-type breast cancer.Cell death and differentiation · 2020Article
- Role of HSP60/HSP10 in Lung Cancer: Simple Biomarkers or Leading Actors?Journal of oncology · 2020Review
- Chemotherapy-induced senescent cancer cells engulf other cells to enhance their survival.The Journal of cell biology · 2019Article
- Induction of accelerated senescence by the microtubule-stabilizing agent peloruside A.Investigational new drugs · 2017Article
- DNA Tetrahedron Delivery Enhances Doxorubicin-Induced Apoptosis of HT-29 Colon Cancer Cells.Nanoscale research letters · 2017Article
- Small molecule compounds that induce cellular senescence.Aging cell · 2016Review
- Formulation of Biologically-Inspired Silk-Based Drug Carriers for Pulmonary Delivery Targeted for Lung Cancer.Scientific reports · 2015Article
- Attenuation of replication stress-induced premature cellular senescence to assess anti-aging modalities.Current protocols in cytometry · 2014Article
- In search of antiaging modalities: evaluation of mTOR- and ROS/DNA damage-signaling by cytometry.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2014Review
- Article
- Novel ferrocenyl derivatives exert anti-cancer effect in human lung cancer cells in vitro via inducing G1-phase arrest and senescence.Acta pharmacologica Sinica · 2013Article
- Ophiobolin A induces paraptosis-like cell death in human glioblastoma cells by decreasing BKCa channel activity.Cell death & disease · 2013Article
- Low-dose etoposide-treatment induces endoreplication and cell death accompanied by cytoskeletal alterations in A549 cells: Does the response involve senescence? The possible role of vimentin.Cancer cell international · 2013Article
- N-acetylcysteine potentiates doxorubicin-induced ATM and p53 activation in ovarian cancer cells.International journal of oncology · 2013Article
- Biomarkers of cell senescence assessed by imaging cytometry.Methods in molecular biology (Clifton, N.J.) · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeSenescence and cell death are fail-safe mechanisms protecting against tumorigenesis. Both these forms of cellular response could be induced in cancer cells, thus suppressing tumor progression. Therefore, to fully understand chemotherapeutic effects, not only symptoms of cell death, but also of senescence should be evaluated. Since the involvement of cytoskeleton components in these processes has been reported, changes in the organization and level of some cytoskeletal proteins may be indicative of cell fate.
methodsWe analyzed selected markers of senescence and cell death, including possible alterations in vimentin and G-actin cytoskeleton in A549 cells after treatment with doxorubicin. Light (SA-beta-galactosidase), fluorescent (vimentin and G-actin labeling) and electron microscopic examinations along with flow cytometry methods (TUNEL, Annexin V/PI staining, cell cycle analysis, intracellular level of vimentin) were employed to determine the outcome of the treatment.
resultsUncoupling between senescent cell morphology and stable cell cycle arrest occurred. Some differences in the organization and level of cytoskeletal proteins, especially of vimentin, like fluctuations in its level, were observed. On the other hand, G-actin seemed to be more stable than vimentin.
conclusionsG-actin stability may imply its potential usefulness for permanent senescence detection. Along with slight to moderate cytoskeletal alterations, the obtained results suggest transient senescence-like state induction, followed by morphology typical of mitotic catastrophe in part of the A549 cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.