ArticleMolecular vision2009
Characterization and short-term culture of cells recovered from human conjunctival epithelium by minimally invasive means.
Article in Molecular vision, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Inhibition of mTOR differently modulates planar and subepithelial fibrogenesis in human conjunctival fibroblasts.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2025Article
- Epithelial component and intraepithelial lymphocytes of conjunctiva-associated lymphoid tissue in healthy children.Histology and histopathology · 2021Article
- Three-Dimensional Human Cell Culture Models to Study the Pathophysiology of the Anterior Eye.Pharmaceutics · 2020Review
- [New approaches to ocular surface reconstruction beyond the cornea].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2017Review
- Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion.Investigative ophthalmology & visual science · 2016Article
- Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.Mucosal immunology · 2016Article
- Concise Review: Comparison of Culture Membranes Used for Tissue Engineered Conjunctival Epithelial Equivalents.Journal of functional biomaterials · 2015Review
- CD44 and RHAMM hyaluronan receptors in human ocular surface inflammation.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2014Article
- Ambient DESI and LESA-MS analysis of proteins adsorbed to a biomaterial surface using in-situ surface tryptic digestion.Journal of the American Society for Mass Spectrometry · 2013Article
- Why we get a blue stain.Eye (London, England) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo characterize conjunctival cells obtained by brush cytology (BC) and establish short-term cultures.
methodsHuman tarsal and bulbar conjunctival cells were obtained by BC and transported in 3 different media: serum-free medium (DK-SFM) with low [Ca(2+)], 10% fetal bovine serum (FBS) supplemented medium (FBSm10), and 20% FBS-supplemented medium (FBSm20). Recovered cells were counted and initial viability assessed. Flow cytometry established epithelial or immune lineage, viability, apoptosis, and cell cycle stage. To establish short-term cultures, tarsal conjunctival cells were seeded onto Permanox or denuded amniotic membrane (dAM) and cultured in the 3 media. Living adherent cells were assessed on Days 1, 2, and 5 by fluorescence microscopy.
resultsInitial cell recovery was significantly lower with DK-SFM than in the other two culture media. Flow cytometry showed that 3.8+/-0.4% of recovered tarsal cells were CD45+ leukocytes and 67.9+/-1.6% were CK7+ secretory epithelial cells. S-phase cells composed 3.5+/-0.3% of the recovered tarsal cells and 2.1+/-0.2% of the bulbar cells (p=0.0006). The percentage of viable, apoptotic, and dead cells was similar for tarsal and bulbar cells. Two different cell populations were observed in both locations. About 24% consisted of smaller, less complex cells with high viability, and the remainder was composed of larger, more complex cells with poor viability. Significantly more living cells were supported by FBSm10 on the dAM substratum (p=0.011) than by the other media on either dAM or Permanox.
conclusionsConjunctival BC recovers proliferating cells that can be maintained on dAM in FBSm10 for up to 5 days.
Indexed as
Identifiers
19898637PMC2773740W2097136639What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.