ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2010
Chromatin condensation modulates access and binding of nuclear proteins.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 91 citations in OpenAlex.
- ZNF512B associates with mitotic spindles, regulates metaphase exit, and is crucial for stem cell differentiation.Nucleic acids research · 2026Article
- The TRIP12's intrinsically disordered region induces chromatin condensates and interferes with nuclear processes.iScience · 2026Article
- Periodic confined cell migration drives partially reversible chromatin reorganization in cancer cell lines.Communications biology · 2026Article
- Nuclear destabilisation - a possible genesis of cancer?Biological reviews of the Cambridge Philosophical Society · 2025Review
- Chromatin unfolding via loops can drive clustered transposon insertion.Biophysical journal · 2025Article
- Pyriproxyfen Disrupts the Ongoing Spermatogenesis Wave in Danio rerio Potentially Mediated by Voltage-Dependent Calcium Channels and Protein Kinase C.Journal of applied toxicology : JAT · 2025Article
- Exposure of cells to near-infrared irradiation relaxes chromatin compaction and facilitates recognition of cyclo-butane pyrimidine dimers.Scientific reports · 2025Article
- Rules of engagement for condensins and cohesins guide mitotic chromosome formation.Science (New York, N.Y.) · 2025Article
- Antiproliferative Effect of Methanolic Extract ofMaterials (Basel, Switzerland) · 2025Article
- Rules of engagement for condensins and cohesins guide mitotic chromosome formation.bioRxiv : the preprint server for biology · 2024Article
- The SMC5/6 complex: folding chromosomes back into shape when genomes take a break.Nucleic acids research · 2024Review
- Epigenetic regulatory layers in the 3D nucleus.Molecular cell · 2024Review
- Live-cell imaging unveils distinct R-loop populations with heterogeneous dynamics.Nucleic acids research · 2023Article
- Evidence for low nanocompaction of heterochromatin in living embryonic stem cells.The EMBO journal · 2023Article
- Convergent and complementary selection shaped gains and losses of eusociality in sweat bees.Nature ecology & evolution · 2023Article
- Visualizing enteric nervous system activity through dye-free dynamic full-field optical coherence tomography.Communications biology · 2023Article
- Adaptive changes in the DNA damage response during skeletal muscle cell differentiation.Frontiers in cell and developmental biology · 2023Article
- Molecular and cellular dynamics of early embryonic cell divisions in Volvox carteri.The Plant cell · 2022Article
- Lysosomal Changes in Mitosis.Cells · 2022Article
- Mitotic chromosomes.Seminars in cell & developmental biology · 2021Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The condensation level of chromatin is controlled by epigenetic modifications and associated regulatory factors and changes throughout differentiation and cell cycle progression. To test whether changes of chromatin condensation levels per se affect access and binding of proteins, we used a hypertonic cell treatment. This shift to hyperosmolar medium increased nuclear calcium concentrations and induced a reversible chromatin condensation comparable to the levels in mitosis. However, this condensation was independent of mitotic histone H3 serine 10 phosphorylation. Photobleaching and photoactivation experiments with chromatin proteins-histone H2B-GFP and GFP-HP1alpha-before and after induced chromatin condensation demonstrated that hypercondensation reduced their dissociation rate and stabilized their chromatin binding. Finally, measuring the distribution of nucleoplasmic proteins in the size range from 30 to 230 kDa, we found that even relatively small proteins like GFP were excluded from highly condensed chromatin in living cells. These results suggest that structural changes in condensed chromatin by themselves affect chromatin access and binding of chromatin proteins independent of regulatory histone modifications.-Martin, R. M., Cardoso, M. C. Chromatin condensation modulates access and binding of nuclear proteins.
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