ArticleJournal of bacteriology2010
The Mtr respiratory pathway is essential for reducing flavins and electrodes in Shewanella oneidensis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 127 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
127 citing papers in PubMed, 472 citations in OpenAlex.
- Menaquinone synthesis genes are critical for the decolorization of acid yellow 36 inMicrobiology spectrum · 2026Article
- Synthetic Shewanella-Bacillus Microbial Consortia for Enhanced Metal Oxide Leaching From Spent Lithium-Ion Batteries Using Brewing Wastewater as Leaching Agent.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Article
- Azurins With Tunable Reduction Potentials as Redox Mediators for Extracellular Electron Transfer.Chembiochem : a European journal of chemical biology · 2026Article
- Deciphering the Mto electron uptake pathway ofApplied and environmental microbiology · 2026Article
- Biosynthesis, Regulation, and Biotechnological Production Strategies of Riboflavin (Vitamin BPharmaceuticals (Basel, Switzerland) · 2026Review
- Flavin Biosynthesis Enhances Extracellular Electron Transfer in Bioengineered Escherichia coli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Disentangling extracellular current of electroactive bacteria with oblique-incidence reflection difference imaging.Nature communications · 2025Article
- Energetic constraints of metal-reducing bacteria as biocatalysts for microbial electrosynthesis.Biotechnology for biofuels and bioproducts · 2025Article
- A Bioelectrochemical Crossbar Architecture Screening Platform (BiCASP) for Extracellular Electron Transfer.bioRxiv : the preprint server for biology · 2025Article
- Iron reduction under oxic conditions by Microbacterium deferre sp. nov. A1-JKNature communications · 2025Article
- A small number of point mutations confer formate tolerance inApplied and environmental microbiology · 2025Article
- Robust measurement of microbial reduction of graphene oxide nanoparticles using image analysis.Applied and environmental microbiology · 2025Article
- Dynamic synthesis and transport of phenazine-1-carboxylic acid to boost extracellular electron transfer rate.Nature communications · 2025Article
- Single-cell phenotyping of extracellular electron transfer via microdroplet encapsulation.Applied and environmental microbiology · 2025Article
- Bioelectricity harvesting from microorganism: review of recent advancements in utilizing the bioelectric properties of fungi for powering small-scale robotic systems.Frontiers in fungal biology · 2025Review
- Shewanella oneidensis: Biotechnological Application of Metal-Reducing Bacteria.Advances in biochemical engineering/biotechnology · 2025Review
- Controlled electron transfer by molecular wires embedded in ultrathin insulating membranes for driving redox catalysis.Photosynthesis research · 2024Review
- Engineered Cell Elongation Promotes Extracellular Electron Transfer of Shewanella Oneidensis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Genetic Code Expansion inACS synthetic biology · 2024Article
67 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The Mtr respiratory pathway of Shewanella oneidensis strain MR-1 is required to effectively respire both soluble and insoluble forms of oxidized iron. Flavins (riboflavin and flavin mononucleotide) recently have been shown to be excreted by MR-1 and facilitate the reduction of insoluble substrates. Other Shewanella species tested accumulated flavins in supernatants to an extent similar to that of MR-1, suggesting that flavin secretion is a general trait of the species. External flavins have been proposed to act as both a soluble electron shuttle and a metal chelator; however, at biologically relevant concentrations, our results suggest that external flavins primarily act as electron shuttles for MR-1. Using deletion mutants lacking various Mtr-associated proteins, we demonstrate that the Mtr extracellular respiratory pathway is essential for the reduction of flavins and that decaheme cytochromes found on the outer surface of the cell (MtrC and OmcA) are required for the majority of this activity. Given the involvement of external flavins in the reduction of electrodes, we monitored current production by Mtr respiratory pathway mutants in three-electrode bioreactors under controlled flavin concentrations. While mutants lacking MtrC were able to reduce flavins at 50% of the rate of the wild type in cell suspension assays, these strains were unable to grow into productive electrode-reducing biofilms. The analysis of mutants lacking OmcA suggests a role for this protein in both electron transfer to electrodes and attachment to surfaces. The parallel phenotypes of Mtr mutants in flavin and electrode reduction blur the distinction between direct contact and the redox shuttling strategies of insoluble substrate reduction by MR-1.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.